Overview

  • Product nameAnti-ACADVL antibodySee all ACADVL primary antibodies ...
  • Description
    Mouse monoclonal to ACADVL
  • Tested applicationsFlow Cyt, WB, IHC-P more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: AAALAGTMRG IIAKAVDHAT NRTQFGEKIH NFGLIQEKLA RMVMLQYVTE SMAYMVSANM DQGATDFQIE AAISKIFGSE AAWKVTDECI , corresponding to amino acids 345-435 of ACADVL Recombinant fragment: AAALAGTMRG IIAKAVDHAT NRTQFGEKIH NFGLIQEKLA RMVMLQYVTE SMAYMVSANM DQGATDFQIE AAISKIFGSE AAWKVTDECI , corresponding to amino acids 345-435 of Human ACADVL

Properties

Applications

Our Abpromise guarantee covers the use of ab54698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt Use 1µg for 106 cells.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
IHC-P Use a concentration of 3 µg/ml.

Target

  • FunctionActive toward esters of long-chain and very long chain fatty acids such as palmitoyl-CoA, mysritoyl-CoA and stearoyl-CoA. Can accomodate substrate acyl chain lengths as long as 24 carbons, but shows little activity for substrates of less than 12 carbons.
  • PathwayLipid metabolism; mitochondrial fatty acid beta-oxidation.
  • Involvement in diseaseDefects in ACADVL are the cause of acyl-CoA dehydrogenase very long chain deficiency (ACADVLD) [MIM:201475]. ACADVLD is an autosomal recessive disease which leads to impaired long-chain fatty acid beta-oxidation. It is clinically heterogeneous, with three major phenotypes: a severe childhood form, with early onset, high mortality, and high incidence of cardiomyopathy; a milder childhood form, with later onset, usually with hypoketotic hypoglycemia as the main presenting feature, low mortality, and rare cardiomyopathy; and an adult form, with isolated skeletal muscle involvement, rhabdomyolysis, and myoglobinuria, usually triggered by exercise or fasting.
  • Sequence similaritiesBelongs to the acyl-CoA dehydrogenase family.
  • Cellular localizationMitochondrion inner membrane.
  • Target information above from: UniProt accession P49748 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • ACAD 6 antibody
    • ACAD6 antibody
    • ACADV_HUMAN antibody
    • Acadvl antibody
    • Acyl CoA dehydrogenase very long chain antibody
    • Acyl Coenzyme A dehydrogenase very long chain antibody
    • LCACD antibody
    • mitochondrial antibody
    • Very long chain specific acyl CoA dehydrogenase antibody
    • Very long chain specific acyl CoA dehydrogenase mitochondrial antibody
    • Very long-chain specific acyl-CoA dehydrogenase antibody
    • VLCAD antibody
    see all

Anti-ACADVL antibody images



  • Predicted band size : 70 kDa
    Observed band size : 70 kDa
  • ab54698 used in immunohistochemistry at 3ug/ml in formalin fixed and paraffin embedded human heart.
  • ICC/IF image of ab54698 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54698, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HeLa cells stained with ab54698 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54698, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-ACADVL antibody (ab54698)

ab54698 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Huh 7.5 cells)
Loading amount 40 µg
Specification Huh 7.5 cells
Gel Running Conditions Reduced Denaturing (12.5% gel)
Blocking step casein as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
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Submitted Aug 27 2009

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