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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Overview
Product name
Anti-ACAT1 antibody - C-terminal
See all ACAT1 products (4) ...
Description
Rabbit polyclonal to ACAT1 - C-terminal
Tested applications
IHC-P, ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide from the C-terminal region of human ACAT1 conjugated to KLH
Positive control
SK-BR-3 cell line lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
ab71407 was prepared by Saturated Ammonium Sulfate precipitation followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71407 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/10 - 1/50.
ELISA
ELISA: 1/1000ELISA: 1/1000
WB: 1/50 - 1/100.Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
Target
Function
Plays a major role in ketone body metabolism.
Involvement in disease
Defects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD) [MIM:203750]; also known as alpha-methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype.
Sequence similarities
Belongs to the thiolase family.
Cellular localization
Mitochondrion.
Target information above from: UniProt accessionP24752
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- ACAT 1 antibody
- ACAT antibody
see all
Database links
- Entrez Gene: 38 Human
- Omim: 607809 Human
- SwissProt: P24752 Human
- Unigene: 232375 Human
Anti-ACAT1 antibody - C-terminal images:
Western blot - ACAT1 antibody - C-terminal (ab71407)
Anti-ACAT1 antibody - C-terminal (ab71407) at 1/60 dilution + SK-BR-3 cell line lysate at 35 µg
Predicted band size : 45 kDa
Observed band size : 45 kDa
Additional bands at : 130 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-ACAT1 antibody - C-terminal(ab71407)
ab71407 at 1/50 dilution staining ACAT1 - C- terminal in human hepatocarcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.
References for Anti-ACAT1 antibody - C-terminal (ab71407)
ab71407 has not yet been referenced specifically in any publications.
Publishing research using ab71407? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"