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Overview

  • Product nameACAT1 protein (His-DHFR tag)See all ACAT1 proteins and peptides ...
  • Protein descriptionRecombinant fragment of Human ACAT1 (amino acids 2-141) with N terminal His-DHFR tag, 14.7kDa (calculated without tag). (UniProt ID = Q96FG8).
  • Uniprot accessionQ96FG8
  • Molecular weight14.700kDa
  • Protein length140 amino acids
  • Expression hostE. coli

    • Properties

  • Purification notesPurity is 70% by SDS-PAGE.
  • FormLyophilised:Reconstitute with water to desired concentration.
  • Storage instructionsShipped at 4°C. Store at -20ºC.
  • Storage bufferConstituents: 0.32% Tris HCl, 0.58% Sodium chloride
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab127352 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        ACATACAT 1acat1
        Acetoacetyl CoA thiolaseAcetoacetyl-CoA thiolaseAcetyl CoA acetyltransferase, mitochondrialAcetyl Coenzyme A acetyltransferase 1Acetyl-CoA acetyltransferaseacetyl-coa acetyltransferase precursor, mitochondrialMATmitochondrialRATACALT2THILTHIL_HUMAN
      see all
  • FunctionPlays a major role in ketone body metabolism.
  • Involvement in diseaseDefects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD) [MIM:203750]; also known as alpha-methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype.
  • Sequence similaritiesBelongs to the thiolase family.
  • Cellular localizationMitochondrion.

    • Target information above from: UniProt accession P24752 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for ACAT1 protein (His-DHFR tag) (ab127352)

    ab127352 has not yet been referenced specifically in any publications.

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