Anti-ACAT2 antibody [7B1] (ab123934)

Overview

• Product nameAnti-ACAT2 antibody [7B1]See all ACAT2 primary antibodies ...
• Description
  Mouse monoclonal [7B1] to ACAT2
• Tested applicationsWB, IHC-P, Flow Cyt, ICC/IF more details
• Species reactivity
  Reacts with: Rat, Dog, Human
  
• Immunogen

  Recombinant full length Human ACAT2 produced in HEK293T cells.

• Positive controlHEK293T cells transfected with pCMV6-ENTRY ACAT2; HepG2, HeLa, HT29, Jurkat, MDCK, PC12, MCF7 cell extracts; Human colon adenocarcinoma, Kidney, Prostate, Prostate carcinoma, Lymph node and lymphoma tissues; COS7 cells transiently transfected by pCMV6-ENTRY ACAT2; HeLa cells.
• General notesDilute in PBS (pH7.3) before use.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.30
  Preservative: 0.02% Sodium azide
  Constituents: 48% PBS, 50% Glycerol, 1% BSA
• Concentration information loading...
• PurityProtein G purified
• Purification notesPurified from Mouse ascites fluid by affinity chromatography.
• Clonality Monoclonal
• Clone number7B1
• IsotypeIgG1
• Research Areas
  • Metabolism
  • Types of disease
  • Heart disease

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Lipoprotein metabolism

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Cholesterol Metabolism

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

  • Cardiovascular
  • Atherosclerosis
  • Lipoprotein metabolism

  • Cardiovascular
  • Lipids / Lipoproteins
  • Lipid Metabolism
  • Cholesterol Metabolism

Applications

Target

• Sequence similaritiesBelongs to the thiolase family.
• Cellular localizationCytoplasm.

Target information above from: UniProt accession Q9BWD1 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 39 Human
  • Entrez Gene: 308100 Rat
  • Omim: 100678 Human
  • SwissProt: Q9BWD1 Human
  • SwissProt: Q5XI22 Rat
  • Unigene: 571037 Human
  • Unigene: 203063 Rat

• Alternative names
  Acat2 antibodyacetoacetyl CoA thiolase antibodyacetoacetyl Coenzyme A thiolase antibody

  Acetyl CoA acetyltransferase antibodyAcetyl CoA transferase like protein antibodyacetyl Coenzyme A acetyltransferase 2 antibodyAcetyl-CoA acetyltransferase antibodyAcetyl-CoA transferase-like protein antibodyAcyl Coenzyme A cholesterol acyltransferase 2 antibodyCytosolic acetoacetyl-CoA thiolase antibodycytosolic antibodySOAT2 antibodyTHIC_HUMAN antibody

  see all

Anti-ACAT2 antibody [7B1] images

• 

  Western blot - Anti-ACAT2 antibody [7B1] (ab123934)
  All lanes : Anti-ACAT2 antibody [7B1] (ab123934) at 1/1000 dilution
  
  Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
  Lane 2 : HEK293T cells transfected with pCMV6-ENTRY ACAT2 cDNA
  
  Lysates/proteins at 5 µg per lane.
  
  
  Predicted band size : 41 kDa
  
• 

  Western blot - Anti-ACAT2 antibody [7B1] (ab123934)
  All lanes : Anti-ACAT2 antibody [7B1] (ab123934) at 1/1000 dilution
  
  Lane 1 : HepG2 cell extract
  Lane 2 : HeLa cell extract
  Lane 3 : HT29 cell extract
  Lane 4 : A549 cell extract
  Lane 5 : COS7 cell extract
  Lane 6 : Jurkat cell extract
  Lane 7 : MDCK cell extract
  Lane 8 : PC12 cell extract
  Lane 9 : MCF7 cell extract
  
  Lysates/proteins at 35 µg per lane.
  
  
  Predicted band size : 41 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT2 antibody [7B1] (ab123934)
  ab123934 at 1/150 dilution staining ACAT2 in Paraffin-embedded Human colon adenocarcinoma tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT2 antibody [7B1] (ab123934)
  ab123934 at 1/150 dilution staining ACAT2 in Paraffin-embedded Human Kidney tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT2 antibody [7B1] (ab123934)
  ab123934 at 1/150 dilution staining ACAT2 in Paraffin-embedded Human Prostate tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT2 antibody [7B1] (ab123934)
  ab123934 at 1/150 dilution staining ACAT2 in Paraffin-embedded Human Prostate carcinoma tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT2 antibody [7B1] (ab123934)
  ab123934 at 1/150 dilution staining ACAT2 in Paraffin-embedded Human Lymph node tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT2 antibody [7B1] (ab123934)
  ab123934 at 1/150 dilution staining ACAT2 in Paraffin-embedded Human Lymphoma tissue by Immunohistochemistry.
• 

  Immunocytochemistry/ Immunofluorescence - Anti-ACAT2 antibody [7B1] (ab123934)
  ab123934 at 1/100 dilution staining ACAT2 in COS7 cells transiently transfected with pCMV6-ENTRY ACAT2 by Immunofluorescence.
• 

  Flow Cytometry - Anti-ACAT2 antibody [7B1] (ab123934)
  ab123934 at 1/100 dilution staining ACAT2 in HeLa cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).