Overview

  • Product nameAnti-ACAT2 antibody [7B1]See all ACAT2 primary antibodies ...
  • Description
    Mouse monoclonal [7B1] to ACAT2
  • Tested applicationsWB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Dog, Human
  • Immunogen

    Recombinant full length Human ACAT2 produced in HEK293T cells.

  • Positive control
    • HEK293T cells transfected with pCMV6-ENTRY ACAT2; HepG2, HeLa, HT29, Jurkat, MDCK, PC12, MCF7 cell extracts; Human colon adenocarcinoma, Kidney, Prostate, Prostate carcinoma, Lymph node and lymphoma tissues; COS7 cells transiently transfected by pCMV6-ENTRY ACAT2; HeLa cells.
  • General notesDilute in PBS (pH7.3) before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab123934 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 41 kDa.
IHC-P 1/150.
Flow Cyt 1/100.
ICC/IF 1/100.

Target

  • Sequence similaritiesBelongs to the thiolase family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acat2 antibody
    • acetoacetyl CoA thiolase antibody
    • acetoacetyl Coenzyme A thiolase antibody
    • Acetyl CoA acetyltransferase antibody
    • Acetyl CoA transferase like protein antibody
    • acetyl Coenzyme A acetyltransferase 2 antibody
    • Acetyl-CoA acetyltransferase antibody
    • Acetyl-CoA transferase-like protein antibody
    • Acyl Coenzyme A cholesterol acyltransferase 2 antibody
    • Cytosolic acetoacetyl-CoA thiolase antibody
    • cytosolic antibody
    • SOAT2 antibody
    • THIC_HUMAN antibody
    see all

Anti-ACAT2 antibody [7B1] images

  • All lanes : Anti-ACAT2 antibody [7B1] (ab123934) at 1/1000 dilution

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY ACAT2 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 41 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC201821)
  • All lanes : Anti-ACAT2 antibody [7B1] (ab123934) at 1/1000 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : HT29 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 41 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC201821)
  • ab123934 at 1/150 dilution staining ACAT2 in Paraffin-embedded Human colon adenocarcinoma tissue by Immunohistochemistry.
  • ab123934 at 1/150 dilution staining ACAT2 in Paraffin-embedded Human Kidney tissue by Immunohistochemistry.
  • ab123934 at 1/150 dilution staining ACAT2 in Paraffin-embedded Human Prostate tissue by Immunohistochemistry.
  • ab123934 at 1/150 dilution staining ACAT2 in Paraffin-embedded Human Prostate carcinoma tissue by Immunohistochemistry.
  • ab123934 at 1/150 dilution staining ACAT2 in Paraffin-embedded Human Lymph node tissue by Immunohistochemistry.
  • ab123934 at 1/150 dilution staining ACAT2 in Paraffin-embedded Human Lymphoma tissue by Immunohistochemistry.
  • ab123934 at 1/100 dilution staining ACAT2 in COS7 cells transiently transfected with pCMV6-ENTRY ACAT2 by Immunofluorescence.
  • ab123934 at 1/100 dilution staining ACAT2 in HeLa cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).

References for Anti-ACAT2 antibody [7B1] (ab123934)

This product has been referenced in:
  • Boschetti E  et al. Influence of genotype on the modulation of gene and protein expression by n-3 LC-PUFA in rats. Genes Nutr 8:589-600 (2013). WB ; Rat . Read more (PubMed: 23744008) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"