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Anti-ACAT2 antibody (ab60282) at 1/2000 dilution + E coli-derived fusion protein
Secondary
Goat anti-IgY-HRP at 1/1000 dilution
Predicted band size : 42 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Colorimetric method used for signal development. The observed band does not match the predicted band size as the test antigen is a fusion protein.
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