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ab29307 |
K562 (Human erythromyeloblastoid leukemia cell line) Cytoplasmic Lysate - tumor cell line (ab29307) |
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Anti-ACAT2 antibody
See all ACAT2 products (5) ...
Rabbit polyclonal to ACAT2
WB, ELISA, IHC-Pmore details
Reacts with
Human
Predicted to work with
Mouse, Cow
Synthetic peptide: SILCDGLTDA FHNCHMGITA ENVAKKWQVS REDQDKVAVL SQNRTENAQK, corresponding to a region within internal sequence amino acids 145-194 of Human ACAT2
SILCDGLTDA FHNCHMGITA ENVAKKWQVS REDQDKVAVL SQNRTENAQK
K562 cell lysate. Human liver tissue.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
Polyclonal
IgG
Our Abpromise guarantee covers the use of ab66259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution. Titre using peptide based assay 1/12500.
IHC-P: Use at a concentration of 4.0 - 8.0 µg/ml.
WB: Use at a concentration of 1.0 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Belongs to the thiolase family.
Cytoplasm.
Target information above from: UniProt accessionQ9BWD1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - ACAT2 antibody (ab66259)

Anti-ACAT2 antibody (ab66259) at 1 µg/ml + K562 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 41 kDa
Observed band size : 41 kDa
Additional bands at : 52 kDa. We are unsure as to the identity of these extra bands.
Antibody diluted in 5% skim milk / PBS buffer. Gel concentration 12%.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ACAT2 antibody (ab66259)

ab66259, at 4.0 µg/ml, staining ACAT2 in Human liver tissue using paraffin-embedded tissue. The arrows indicate positively stained hepatocytes.
ab66259 has not yet been referenced specifically in any publications.
Publishing research using ab66259? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:

Anti-ACAT2 antibody (ab66259) at 1 µg/ml + K562 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 41 kDa
Observed band size : 41 kDa
Additional bands at : 52 kDa. We are unsure as to the identity of these extra bands.
Antibody diluted in 5% skim milk / PBS buffer. Gel concentration 12%.

ab66259, at 4.0 µg/ml, staining ACAT2 in Human liver tissue using paraffin-embedded tissue. The arrows indicate positively stained hepatocytes.
0
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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