Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Overview
Product name
Anti-ACAT2 antibody
See all ACAT2 products (5) ...
Description
Rabbit polyclonal to ACAT2
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Cow
Immunogen
Synthetic peptide: SILCDGLTDA FHNCHMGITA ENVAKKWQVS REDQDKVAVL SQNRTENAQK, corresponding to a region within internal sequence amino acids 145-194 of Human ACAT2
SILCDGLTDA FHNCHMGITA ENVAKKWQVS REDQDKVAVL SQNRTENAQK
Positive control
K562 cell lysate. Human liver tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab66259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ELISA: Use at an assay dependent dilution. Titre using peptide based assay 1/12500.
IHC-P: Use at a concentration of 4.0 - 8.0 µg/ml.
WB: Use at a concentration of 1.0 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Sequence similarities
Belongs to the thiolase family.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ9BWD1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- cytosolic antibody
- Acat2 antibody
- acetoacetyl CoA thiolase antibody
see all
Database links
- Entrez Gene: 512044 Cow
- Entrez Gene: 39 Human
- Entrez Gene: 110460 Mouse
- Omim: 100678 Human
- SwissProt: Q17QI3 Cow
- SwissProt: Q1JPB6 Cow
- SwissProt: Q9BWD1 Human
- SwissProt: Q8CAY6 Mouse
see all
Anti-ACAT2 antibody images:
Western blot - ACAT2 antibody (ab66259)
Anti-ACAT2 antibody (ab66259) at 1 µg/ml + K562 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 41 kDa
Observed band size : 41 kDa
Additional bands at : 52 kDa. We are unsure as to the identity of these extra bands.
Antibody diluted in 5% skim milk / PBS buffer. Gel concentration 12%.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ACAT2 antibody (ab66259)
ab66259, at 4.0 µg/ml, staining ACAT2 in Human liver tissue using paraffin-embedded tissue. The arrows indicate positively stained hepatocytes.
References for Anti-ACAT2 antibody (ab66259)
ab66259 has not yet been referenced specifically in any publications.
Publishing research using ab66259? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"