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Products:Neuroscience >> Neurotransmission >> Receptors / Channels >> Sodium Channels
Overview
Product name
Anti-ACCN2 antibody - Extracellular domain
See all ACCN2 products (4) ...
Description
Sheep polyclonal to ACCN2 - Extracellular domain
Specificity
ab81058 is specific for ACCN2.
Tested applications
WB, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide from the extracellular domain of rat ACCN2 conjugated to an immunogenic carrier protein.
Properties
Form
Lyophilised:
Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurotransmission >> Receptors / Channels >> Sodium Channels
Applications
Show applications key
Our Abpromise guarantee covers the use of ab81058 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 10 - 50 µg/ml.Predicted molecular weight: 60 kDa.(This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.)
IHC-P: Use a concentration of 10 - 50 µg/ml.
IHC-Fr: Use a concentration of 10 - 50 µg/ml.
Target
Function
Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Ca(2+), Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear.
Tissue specificity
Expressed in most or all neurons.
Sequence similarities
Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ACCN2 subfamily.
Post-translational
modifications
Phosphorylation by PKA regulates interaction with PRKCABP and subcellular location. Phosphorylation by PKC may regulate the channel.
Cellular localization
Cell membrane. Localizes in synaptosomes at dendritic synapses of neurons. Colocalizes with DLG4.
Target information above from: UniProt accessionP78348
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- neuronal antibody
- ACCN2 antibody
- ACCN2_HUMAN antibody
see all
Database links
- Entrez Gene: 41 Human
- Entrez Gene: 11419 Mouse
- Entrez Gene: 79123 Rat
- Omim: 602866 Human
- SwissProt: P78348 Human
- SwissProt: Q6NXK8 Mouse
- SwissProt: P55926 Rat
- Unigene: 274361 Human
see all
References for Anti-ACCN2 antibody - Extracellular domain (ab81058)
ab81058 has not yet been referenced specifically in any publications.
Publishing research using ab81058? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"