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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ACCN2 antibody (ab65698)
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Product Name
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ACCN2 antibody
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See all ACCN2 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ACCN2
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Immunogen
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A synthetic peptide to an extracellular domain of human and rat ACCN2 conjugated to an immunogenic carrier protein.
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Reacts with
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Hu, Rat
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Specificity
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This antibody recognizes both subtype 1a and 1b.
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Tested applications
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IHC-Fr, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: 1/300 - 1/4000.
Note: antigen retrieval step is recommended.
IHC-Fr: 1/300 - 1/4000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cell Membrane; Multi-pass membrane protein
Note: Localizes in synaptosomes at dendritic synapses of neurons. Co-localizes with DLG4.
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Research areas
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Neuroscience >> Neurotransmission >> Receptors / Channels >> Sodium Channels
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Relevance
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ACCN2 belongs to the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. ACCN2 is expressed in most if not all brain neurons, and it may be an ion channel subunit; however, its function as an ion channel remains unknown. Alternative splicing of this gene generates 2 transcript products.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: None
Constituents: Whole serum
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Form
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Lyophilised
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ACCN2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ACCN2 antibody images, references, products related to ab65698 and other tools.
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ACCN2 antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ACCN2 antibody (ab65698)
Ab65698 staining Human frontal lateral cortex. Staining is localised to the cell membrane.
Left panel: with primary antibody diluted 1:2000. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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Search PubMed (MEDLINE) for references to ACCN2
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ACCN2 antibody - more information
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Customer reviews (feedback) regarding ACCN2 antibody |
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ACCN2 antibody - related products:
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ACCN2 antibody - other tools:
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