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Products:Neuroscience >> Neurotransmission >> Receptors / Channels >> Sodium Channels
Overview
Product name
Anti-ACCN2 antibody
See all ACCN2 products (4) ...
Description
Rabbit polyclonal to ACCN2
Specificity
This antibody recognizes both subtype 1a and 1b.
Tested applications
IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Rat, Human
Immunogen
A synthetic peptide to an extracellular domain of human and rat ACCN2 conjugated to an immunogenic carrier protein.
Properties
Form
Lyophilised:
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Concentration
Concentration information loading...
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurotransmission >> Receptors / Channels >> Sodium Channels
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ACCN2 antibody (ab65698)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab65698 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/300 - 1/4000.
IHC-Fr
IHC-Fr: 1/300 - 1/4000.IHC-Fr: 1/300 - 1/4000.
Target
Function
Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Ca(2+), Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear.
Tissue specificity
Expressed in most or all neurons.
Sequence similarities
Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ACCN2 subfamily.
Post-translational
modifications
Phosphorylation by PKA regulates interaction with PRKCABP and subcellular location. Phosphorylation by PKC may regulate the channel.
Cellular localization
Cell membrane. Localizes in synaptosomes at dendritic synapses of neurons. Colocalizes with DLG4.
Target information above from: UniProt accessionP78348
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- neuronal antibody
- ACCN2 antibody
- ACCN2_HUMAN antibody
see all
Database links
- Entrez Gene: 41 Human
- Entrez Gene: 79123 Rat
- Omim: 602866 Human
- SwissProt: P78348 Human
- SwissProt: P55926 Rat
- Unigene: 274361 Human
- Unigene: 37385 Rat
Anti-ACCN2 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ACCN2 antibody (ab65698)
Ab65698 staining Human frontal lateral cortex. Staining is localised to the cell membrane.
Left panel: with primary antibody diluted 1:2000. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
References for Anti-ACCN2 antibody (ab65698)
ab65698 has not yet been referenced specifically in any publications.
Publishing research using ab65698? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"