Overview
Product nameACCN2 proteinSee all ACCN2 proteins and peptides ...
Protein descriptionRecombinant full length Human ACCN2 (amino acids 1-528) with N terminal proprietary tag; Predicted MWt 84.15 kDa (UniProt P78348).
Uniprot accessionP78348
Molecular weight84.150kDa inclusive of tags
Protein length528 amino acids
Expression hostWheat germ
Properties
FormLiquid
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 8.00
Constituents: 0.3% Glutathione, 0.79% Tris HCl
Concentration information loading...
Additional notesProtein concentration is above or equal to 0.05 mg/ml.
Best used within three months from the date of receipt.
Sequence notesMELKAEEEEVGGVQPVSIQAFASSSTLHGLAHIFSYERLS LKRALWALCFLGSLAVLLCVCTERVQYYFHYHHVTKLDEV AASQLTFPAVTLCNLNEFRFSQVSKNDLYHAGELLALLNN RYEIPDTQMADEKQLEILQDKANFRSFKPKPFNMREFYDR AGHDIRDMLLSCHFRGEVCSAEDFKVVFTRYGKCYTFNSG RDGRPRLKTMKGGTGNGLEIMLDIQQDEYLPVWGETDETS FEAGIKVQIHSQDEPPFIDQLGFGVAPGFQTFVACQEQRL IYLPPPWGTCKAVTMDSDLDFFDSYSITACRIDCETRYLV ENCNCRMVHMPGDAPYCTPEQYKECADPALDFLVEKDQEY CVCEMPCNLTRYGKELSMVKIPSKASAKYLAKKFNKSEQY IGENILVLDIFFEVLNYETIEQKKAYEIAGLLGDIGGQMG LFIGASILTVLELFDYAYEVIKHKLCRRGKCQKEAKRSSA DKGVALSLDDVKRHNPCESLRGHPAGMTYAANILPHHPAR GTFEDFTC
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab114681
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
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Notes |
| WB |
WB: Use at an assay dependent concentration. |
| SDS-PAGE |
SDS-PAGE: Use at an assay dependent concentration. |
| ELISA |
ELISA: Use at an assay dependent concentration. |
Protein info
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Alternative names
ACCN2ACCN2_HUMANAcid sensing ion channel
Acid sensing ion channel 1Acid sensing ion channel 1a proteinAcid-sensing ion channel 1AI843610Amiloride sensitive cation channel 2 neuronalAmiloride-sensitive cation channel 2ASICASIC1ASIC1AB530003N02RikBNaC2Brain sodium channel 2Cation channel amiloride sensitive neuronal 2hBNaC2neuronal
see all
FunctionCation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Ca(2+), Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear.
Tissue specificityExpressed in most or all neurons.
Sequence similaritiesBelongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ACCN2 subfamily.
Post-translational
modificationsPhosphorylation by PKA regulates interaction with PRKCABP and subcellular location. Phosphorylation by PKC may regulate the channel.
Cellular localizationCell membrane. Localizes in synaptosomes at dendritic synapses of neurons. Colocalizes with DLG4.
References for ACCN2 protein (ab114681)
ab114681
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
SDS-PAGE - ACCN2 protein (ab114681)ab114681 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.