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Anti-ACK1 antibody (ab37367)

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Overview

Product name

Anti-ACK1 antibody
See all ACK1 products (7) ...

Description

Chicken polyclonal to ACK1

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Immunogen corresponds to amino acids 710-933 of Human ACK1

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgY

Research areas

Cancer >> Growth factors >> EGF
Cancer >> Cell cycle >> Kinases/phosphatases >> Other
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Testosterone
Signal Transduction >> Growth Factors/Hormones >> EGF
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other

Applications

Show applications key

Our Abpromise guarantee covers the use of ab37367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide

    WB

     WB: 1/2000. Predicted mo...Read more →

    WB: 1/2000. Predicted molecular weight: 114 kDa.Use 2.5 µg protein in the colorimetric method and about 10x less for ECL.

Target

Function

Non-receptor tyrosine kinase that regulates the activity of a number of proteins by tyrosine phosphorylation especially proteins critical for cell survival, cell growth, and proliferation. Activates AKT1 by phosphorylating it on 'Tyr-176' resulting in its activation. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' and promotes its recruitment to the androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR. Participates in clathrin-mediated endocytosis. May be involved both in adult synaptic function and plasticity and in brain development.

Tissue specificity

The Tyr-284 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages. It also shows a significant increase in expression in prostate cancers during the progressive stages.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
Contains 1 SH3 domain.
Contains 1 UBA domain.

Domain

The EBD (EGFR-binding domain) domain is necessary for interaction with EGFR.
The SAM-like domain is necessary for NEDD4-mediated ubiquitination. Promotes membrane localization and dimerization to allow for autophosphorylation.
The UBA domain binds both poly- and mono-ubiquitin.

Post-translational
modifications

Autophosphorylation regulates kinase activity. Phosphorylation on Tyr-518 is required for interaction with SRC.
Ubiquitinated by NEDD4. Its EGF-induced degradation is EGFR activation-dependent and is processed by lysosomes, not proteasomes.

Cellular localization

Cell membrane. Nucleus. Endosome. Cell junction > adherens junction. Cytoplasmic vesicle membrane. The Tyr-284 phosphorylated form is expressed both in the membrane and nucleus. Co-localizes with EGFR on the endosomes.

Target information above from: UniProt accessionQ07912 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Acetate kinase 1 antibody
  • Acetokinase 1 antibody
  • ACK 1 antibody
  • ACK antibody
  • ACK-1 antibody
  • ACK1_HUMAN antibody
  • Activated Cdc42 associated kinase 1 antibody
  • Activated CDC42 kinase 1 antibody
  • Activated p21cdc42Hs kinase antibody
  • EC 2.7.10.2 antibody
  • FLJ44758 antibody
  • FLJ45547 antibody
  • p21cdc42Hs antibody
  • TNK 2 antibody
  • TNK2 antibody
  • Tyrosine kinase non receptor 2 antibody
  • Tyrosine kinase non receptor protein 2 antibody
  • Tyrosine kinase non-receptor protein 2 antibody
see all

Database links

References for Anti-ACK1 antibody (ab37367)

ab37367 has not yet been referenced specifically in any publications.

Publishing research using ab37367? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"