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Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab77694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/500 - 1/1000. Predicted molecular weight: 114 kDa.

Target

  • FunctionNon-receptor tyrosine kinase that regulates the activity of a number of proteins by tyrosine phosphorylation especially proteins critical for cell survival, cell growth, and proliferation. Activates AKT1 by phosphorylating it on 'Tyr-176' resulting in its activation. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' and promotes its recruitment to the androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR. Participates in clathrin-mediated endocytosis. May be involved both in adult synaptic function and plasticity and in brain development.
  • Tissue specificityThe Tyr-284 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages. It also shows a significant increase in expression in prostate cancers during the progressive stages.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
    Contains 1 SH3 domain.
    Contains 1 UBA domain.
  • DomainThe EBD (EGFR-binding domain) domain is necessary for interaction with EGFR.
    The SAM-like domain is necessary for NEDD4-mediated ubiquitination. Promotes membrane localization and dimerization to allow for autophosphorylation.
    The UBA domain binds both poly- and mono-ubiquitin.
  • Post-translational
    modifications    Autophosphorylation regulates kinase activity. Phosphorylation on Tyr-518 is required for interaction with SRC.
    Ubiquitinated by NEDD4. Its EGF-induced degradation is EGFR activation-dependent and is processed by lysosomes, not proteasomes.
  • Cellular localizationCell membrane. Nucleus. Endosome. Cell junction > adherens junction. Cytoplasmic vesicle membrane. The Tyr-284 phosphorylated form is expressed both in the membrane and nucleus. Co-localizes with EGFR on the endosomes.

    • Target information above from: UniProt accession Q07912 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      Acetate kinase 1 antibodyAcetokinase 1 antibodyACK 1 antibody
      ACK antibodyACK-1 antibodyACK1 antibodyACK1_HUMAN antibodyActivated Cdc42 associated kinase 1 antibodyActivated CDC42 kinase 1 antibodyActivated p21cdc42Hs kinase antibodyFLJ44758 antibodyFLJ45547 antibodyp21cdc42Hs antibodyTNK 2 antibodyTNK2 antibodyTyrosine kinase non receptor 2 antibodyTyrosine kinase non receptor protein 2 antibodyTyrosine kinase non-receptor protein 2 antibody
    see all

Anti-ACK1 antibody images

  • Western blot - ACK1 antibody (ab77694)
    Western blot - ACK1 antibody (ab77694)
    Anti-ACK1 antibody (ab77694) at 1/1000 dilution + PC-12 cell RIPA lysate

    Secondary
    HRP conjugated goat anti-rabbit
    developed using the ECL technique

    Predicted band size : 114 kDa
    Observed band size : 114 kDa

References for Anti-ACK1 antibody (ab77694)

ab77694 has not yet been referenced specifically in any publications.

Publishing research using ab77694 ? Please let us know so that we can cite the reference in this datasheet

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