ACK1 protein (Active) (ab89747)
Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5
Our Abpromise guarantee covers the use of ab89747 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|SDS-PAGE||SDS-PAGE: Use at an assay dependent dilution.|
|WB||WB: Use at an assay dependent dilution.|
|Functional Studies||FuncS: Use at an assay dependent dilution.|
- Acetate kinase 1Acetokinase 1ACK
- ACK 1ACK-1ACK1ACK1_HUMANActivated Cdc42 associated kinase 1Activated CDC42 kinase 1Activated p21cdc42Hs kinaseFLJ44758FLJ45547p21cdc42HsTNK 2TNK2Tyrosine kinase non receptor 2Tyrosine kinase non receptor protein 2Tyrosine kinase non-receptor protein 2
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
Contains 1 SH3 domain.
Contains 1 UBA domain.
The SAM-like domain is necessary for NEDD4-mediated ubiquitination. Promotes membrane localization and dimerization to allow for autophosphorylation.
The UBA domain binds both poly- and mono-ubiquitin.
modificationsAutophosphorylation regulates kinase activity. Phosphorylation on Tyr-518 is required for interaction with SRC.
Ubiquitinated by NEDD4. Its EGF-induced degradation is EGFR activation-dependent and is processed by lysosomes, not proteasomes.
ACK1 protein (Active) images
SDS-PAGE showing ab89747 at approximately 66kDa.
Kinase Assay demonstrating specific activity of ab89747.
References for ACK1 protein (Active) (ab89747)
ab89747 has not yet been referenced specifically in any publications.