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Overview

  • Product nameACK1 protein (Active)See all ACK1 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 110-476 of Human ACK1 with N-terminal proprietary tag expressed in a Baculovirus infected Sf9 cell expression system, 66kDa. UniProt = Q07912.
  • Expression hostBaculovirus infected sf9 cells

    • Properties

  • Purification notesPurity was determined to be >95% by densitometry.
  • Biological activityThe Specific activity of ab89747 was determined to be 20 nmol/min/mg.
  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab89747 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
    WB WB: Use at an assay dependent dilution.
    Functional Studies FuncS: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        Acetate kinase 1Acetokinase 1ACK
        ACK 1ACK-1ACK1ACK1_HUMANActivated Cdc42 associated kinase 1Activated CDC42 kinase 1Activated p21cdc42Hs kinaseFLJ44758FLJ45547p21cdc42HsTNK 2TNK2Tyrosine kinase non receptor 2Tyrosine kinase non receptor protein 2Tyrosine kinase non-receptor protein 2
      see all
  • FunctionNon-receptor tyrosine kinase that regulates the activity of a number of proteins by tyrosine phosphorylation especially proteins critical for cell survival, cell growth, and proliferation. Activates AKT1 by phosphorylating it on 'Tyr-176' resulting in its activation. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' and promotes its recruitment to the androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR. Participates in clathrin-mediated endocytosis. May be involved both in adult synaptic function and plasticity and in brain development.
  • Tissue specificityThe Tyr-284 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages. It also shows a significant increase in expression in prostate cancers during the progressive stages.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
    Contains 1 SH3 domain.
    Contains 1 UBA domain.
  • DomainThe EBD (EGFR-binding domain) domain is necessary for interaction with EGFR.
    The SAM-like domain is necessary for NEDD4-mediated ubiquitination. Promotes membrane localization and dimerization to allow for autophosphorylation.
    The UBA domain binds both poly- and mono-ubiquitin.
  • Post-translational
    modifications    Autophosphorylation regulates kinase activity. Phosphorylation on Tyr-518 is required for interaction with SRC.
    Ubiquitinated by NEDD4. Its EGF-induced degradation is EGFR activation-dependent and is processed by lysosomes, not proteasomes.
  • Cellular localizationCell membrane. Nucleus. Endosome. Cell junction > adherens junction. Cytoplasmic vesicle membrane. The Tyr-284 phosphorylated form is expressed both in the membrane and nucleus. Co-localizes with EGFR on the endosomes.

    • Target information above from: UniProt accession Q07912 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    ACK1 protein (Active) images

    • SDS-PAGE - ACK1 protein (Active) (ab89747)
      SDS-PAGE - ACK1 protein (Active) (ab89747)
      SDS-PAGE showing ab89747 at approximately 66kDa.
    • Functional Studies - ACK1 protein (Active) (ab89747)
      Functional Studies - ACK1 protein (Active) (ab89747)
      Kinase Assay demonstrating specific activity of ab89747.

    References for ACK1 protein (Active) (ab89747)

    ab89747 has not yet been referenced specifically in any publications.

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