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Products:Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
Overview
Product name
Anti-ACOT7 antibody
See all ACOT7 products (3) ...
Description
Mouse polyclonal to ACOT7
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Full length ACOT7 protein (Human).(NP_863654.1, 380 a.a).
Positive control
ACOT7 transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Regulation
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
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Western blot - ACOT7 antibody (ab68560)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab68560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 47 kDa (predicted molecular weight: 42 kDa).
Target
Function
Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. May play an important physiological function in brain. May play a regulatory role by modulating the cellular levels of fatty acyl-CoA ligands for certain transcription factors as well as the substrates for fatty acid metabolizing enzymes, contributing to lipid homeostasis. Has broad specificity, active towards fatty acyl-CoAs with chain-lengths of C8-C18. Has a maximal activity toward palmitoyl-CoA.
Tissue specificity
Isoform 4 is expressed exclusively in brain.
Sequence similarities
Contains 2 acyl coenzyme A hydrolase domains.
Cellular localization
Cytoplasm and Mitochondrion.
Target information above from: UniProt accessionO00154
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ACH1 antibody
- ACOT 7 antibody
- ACOT7 antibody
see all
Database links
- Entrez Gene: 11332 Human
- Omim: 602587 Human
- SwissProt: O00154 Human
- Unigene: 126137 Human
Anti-ACOT7 antibody images:
Western blot - ACOT7 antibody (ab68560)
All lanes : Anti-ACOT7 antibody (ab68560) at 1/500 dilution
Lane 1 : ACOT7 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 42 kDa
Observed band size : 47 kDa (why is the actual band size different from the predicted?)
References for Anti-ACOT7 antibody (ab68560)
ab68560 has not yet been referenced specifically in any publications.
Publishing research using ab68560? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"