Anti-ACSL1 antibody (ab189939)
Key features and details
- Rabbit polyclonal to ACSL1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ACSL1 antibody
See all ACSL1 primary antibodies -
Description
Rabbit polyclonal to ACSL1 -
Host species
Rabbit -
Specificity
At least three isoforms of ACSL1 are known to exist; ab189939 will detect all three isoforms. -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human ACSL1 (internal sequence). The exact sequence is proprietary. The peptide corresponds to 18 amino acids. (NCBI Reference Sequence: NP_001986.2)
Database link: P33121 -
Positive control
- Human kidney tissue, Human lung tissue and lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 µg/ml.
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ICC/IF |
Use a concentration of 20 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 78 kDa.
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Notes |
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IHC-P
Use a concentration of 5 µg/ml. |
ICC/IF
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 78 kDa. |
Target
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Function
Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate. -
Tissue specificity
Highly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas. -
Sequence similarities
Belongs to the ATP-dependent AMP-binding enzyme family. -
Developmental stage
Expressed during the early stages of erythroid development while expression is very low in reticulocytes and young erythrocytes. -
Cellular localization
Mitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 2180 Human
- Omim: 152425 Human
- SwissProt: P33121 Human
- Unigene: 406678 Human
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Alternative names
- ACS 1 antibody
- ACS1 antibody
- ACSL 1 antibody
see all
Images
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Lane 1 : Anti-ACSL1 antibody (ab189939) at 1 µg
Lane 2 : Anti-ACSL1 antibody (ab189939) at 2 µg
All lanes : Human lung tissue
Predicted band size: 78 kDa -
Immunofluorescent analysis of Human lung tissue, staining ACSL1 with ab189939 at 20 µg/ml.
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Immunohistochemistry of formalin-fixed, paraffin-embedded Human kidney tissue labeling ACSL1 with ab189939 at 5 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab189939 has not yet been referenced specifically in any publications.