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Anti-ACSL3 antibody (ab80675)

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Overview

Product name

Anti-ACSL3 antibody
See all ACSL3 products (3) ...

Description

Rabbit polyclonal to ACSL3

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Cow, Pig, Orangutan

Immunogen

Synthetic peptide: NSLDGLASVL YPGCDTLDKV FTYAKNKFKN KRLLGTREVL NEEDEVQPNG , corresponding to N terminal amino acids 72-121 of Human ACSL3

NSLDGLASVL YPGCDTLDKV FTYAKNKFKN KRLLGTREVL NEEDEVQPNG

Positive control

293T cell lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - ACSL3 antibody (ab80675)Western blot - ACSL3 antibody (ab80675) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab80675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 1 µg/mlDetects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).

Target

Relevance

ACSL3 (long-chain fatty-acid-Coenzyme A ligase 3) is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates.

Cellular localization

Mitochondrion outer membrane; Single-pass type III membrane protein (By similarity). Peroxisome membrane; Single-pass type III membrane protein (By similarity). Microsome membrane; Single-pass type III membrane protein (By similarity).

Alternative names

  • EC 6.2.1.3 antibody
  • LACS 3 antibody
  • Long chain acyl CoA synthetase 3 antibody
  • Long chain fatty acid CoA ligase 3 antibody

Anti-ACSL3 antibody images:

  Western blot - ACSL3 antibody (ab80675)

Western blot - ACSL3 antibody (ab80675)

Anti-ACSL3 antibody (ab80675) at 1 µg/ml + 293T cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 80 kDa
Observed band size : 80 kDa
Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

Gel concentration: 12%

References for Anti-ACSL3 antibody (ab80675)

ab80675 has not yet been referenced specifically in any publications.

Publishing research using ab80675? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"