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Western blot - ACSL3 antibody (ab80675)
(enlarge)
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Product Name
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ACSL3 antibody
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See all ACSL3 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ACSL3
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Immunogen
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Synthetic peptide: NSLDGLASVL YPGCDTLDKV FTYAKNKFKN KRLLGTREVL NEEDEVQPNG , corresponding to N terminal amino acids 72-121 of Human ACSL3
BLAST 'NSLDGLASVL YPGCDTLDKV FTYAKNKFKN KRLLGTREVL NEEDEVQPNG ' with  or 
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Reacts with
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Hu, Ms
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Predicted to react
(species key)
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Rat, Cow, Pig, Orngt
(Due to sequence homology)
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 80 kDa.
ELISA titre using peptide based assay: 1:312500
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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293T cell lysate.
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Cellular localization
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Mitochondrion outer membrane; Single-pass type III membrane protein (By similarity). Peroxisome membrane; Single-pass type III membrane protein (By similarity). Microsome membrane; Single-pass type III membrane protein (By similarity).
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Research areas
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Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
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Relevance
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ACSL3 (long-chain fatty-acid-Coenzyme A ligase 3) is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ACSL3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ACSL3 antibody images, references, products related to ab80675 and other tools.
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ACSL3 antibody images:
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 Western blot - ACSL3 antibody (ab80675)
ACSL3 antibody (ab80675) at 1 µg/ml + 293T cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 80 kDa
Observed band size : 80 kDa
Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
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Search PubMed (MEDLINE) for references to ACSL3
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ACSL3 antibody - more information
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Customer reviews (feedback) regarding ACSL3 antibody |
Customer FAQs regarding ACSL3 antibody |
Protocols for ACSL3 antibody |
Price and availability of products related to ACSL3 antibody |
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ACSL3 antibody - related products:
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ACSL3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ACSL3 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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