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Overview

  • Product nameACSL3 protein (Tagged-His Tag)
  • Protein descriptionRecombinant fragment, corresponding to amino acids 535-720 (186 amino acids) of Human ACSL3 with an N terminal His tag; MWt 25 kDa (22 kDa predicted from sequence) UniProt O95573.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 91 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesVTMGYYKNEAKTKADFFEDENGQRWLCTGDIGEFEPDGCL KIIDRKKD LVKLQAGEYVSLGKVEAALKNLPLVDNICA YANSYHSYVIGFVVPNQK ELTELARKKGLKGTWEELCN SCEMENEVLKVLSEAAISASLEKFEIPV KIRLSPEPWT PETGLVTDAFKLKRKELKTHYQADIERMYGRK
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91913 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    MS
    SDS-PAGE
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        ACS3Acsl3ACSL3_HUMAN
        Acyl CoA synthetase long chain family member 3EC 6.2.1.3FACL3Fatty acid Coenzyme A ligase long chain 3LACS 3LACS3Lignoceroyl CoA synthaseLong chain acyl CoA synthetase 3Long chain fatty acid CoA ligase 3Long-chain acyl-CoA synthetase 3Long-chain-fatty-acid--CoA ligase 3PRO2194
      see all
  • FunctionAcyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis (By similarity). Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates (By similarity). Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins).
  • Sequence similaritiesBelongs to the ATP-dependent AMP-binding enzyme family.
  • Cellular localizationMitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane.

    • Target information above from: UniProt accession O95573 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    ACSL3 protein (Tagged-His Tag) images

    • SDS-PAGE - ACSL3 protein (Tagged-His Tag) (ab91913)
      SDS-PAGE - ACSL3 protein (Tagged-His Tag) (ab91913)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for ACSL3 protein (Tagged-His Tag) (ab91913)

    ab91913 has not yet been referenced specifically in any publications.

    Publishing research using ab91913 ? Please let us know so that we can cite the reference in this datasheet

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