Anti-ACTL6A antibody (ab189315)
Key features and details
- Rabbit polyclonal to ACTL6A
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ACTL6A antibody
See all ACTL6A primary antibodies -
Description
Rabbit polyclonal to ACTL6A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cynomolgus monkey -
Immunogen
Synthetic peptide corresponding to Human ACTL6A aa 200-300.
Database link: O96019 -
Positive control
- Human skin tissue; HUVEC cells; Jurkat and COLO205 cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS without Mg2+, Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189315 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 47 kDa.
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IHC-P |
Use a concentration of 20 µg/ml.
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ICC/IF |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 47 kDa. |
IHC-P
Use a concentration of 20 µg/ml. |
ICC/IF
1/100 - 1/500. |
Target
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Function
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for maximal ATPase activity of SMARCA4/BRG1/BAF190A and for association of the SMARCA4/BRG1/BAF190A containing remodeling complex BAF with chromatin/nuclear matrix. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Putative core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. -
Sequence similarities
Belongs to the actin family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 86 Human
- Omim: 604958 Human
- SwissProt: Q4R333 Cynomolgus monkey
- SwissProt: O96019 Human
- Unigene: 435326 Human
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Alternative names
- 53 kDa BRG1 associated factor A antibody
- 53 kDa BRG1-associated factor A antibody
- ACL6A_HUMAN antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue, labeling ACTL6A with ab189315 at 20 µg/ml.
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All lanes : Anti-ACTL6A antibody (ab189315) at 1/500 dilution
Lane 1 : Jurkat cell extracts
Lane 2 : COLO205 cell extracts
Lane 3 : Cell extracts treated with synthesized peptide.
Predicted band size: 47 kDa -
Immunofluorescent analysis of HUVEC cells labeling ACTL6A with ab189315 at 1/100 dilution. The picture on the right is treated with the synthesized peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab189315 has not yet been referenced specifically in any publications.