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Anti-ACTN3 antibody (ab65067)

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Overview

Product name

Anti-ACTN3 antibody
See all ACTN3 products (3) ...

Description

Rabbit polyclonal to ACTN3

Tested applications

ELISA, WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat

Immunogen

Synthetic peptide derived from N-terminus of human ACTN3.

Positive control

Extracts from HT-29 cells

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Crosslinking

  • Western blot - ACTN3 antibody (ab65067)Western blot - ACTN3 antibody (ab65067) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab65067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     ELISA: 1/20000

    ELISA: 1/20000

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Detec...Read more →

    WB: 1/500 - 1/1000.Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).

Target

Function

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

Tissue specificity

Expressed only in a subset of type 2 skeletal muscle fibers.

Sequence similarities

Belongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats.

Target information above from: UniProt accessionQ08043 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • actinin, alpha antibody
  • Actinin, alpha 3 antibody
  • ACTN 3 antibody
  • ACTN3 antibody
  • ACTN3_HUMAN antibody
  • Alpha actinin skeletal muscle antibody
  • Alpha actinin skeletal muscle isoform 3 antibody
  • Alpha-Actinin 3 antibody
  • Alpha-actinin skeletal muscle isoform 3 antibody
  • Alpha-actinin-3 antibody
  • F actin cross linking protein antibody
  • F-actin cross-linking protein antibody
  • MGC117002 antibody
  • MGC117005 antibody
  • OTTHUMP00000219049 antibody
  • OTTHUMP00000219054 antibody
see all

Database links

see all

Anti-ACTN3 antibody images:

  Western blot - ACTN3 antibody (ab65067)

Western blot - ACTN3 antibody (ab65067)

All lanes : Anti-ACTN3 antibody (ab65067) at 1/500 dilution

Lane 1 : extracts from HT-29 cells
Lane 2 : extracts from HT-29 cells with immunizing peptide at 5 µg

Lysates/proteins at 5 µg per lane.


Predicted band size : 103 kDa
Observed band size : 103 kDa

References for Anti-ACTN3 antibody (ab65067)

ab65067 has not yet been referenced specifically in any publications.

Publishing research using ab65067? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"