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Western blot - ACTN3 antibody (ab65067)

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Product Name
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ACTN3 antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ACTN3
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Immunogen
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Synthetic peptide derived from N-terminus of human ACTN3.
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ELISA: 1/20000. WB: 1/500 - 1/1000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Extracts from HT-29 cells
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Cellular localization
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actin filament
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Research areas
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Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Crosslinking
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Relevance
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ACTN3 (Alpha-actinin) is an actin-binding protein and is expressed only in a subset of type 2 skeletal muscle fibers. It is localized to the Z-disc and analogous dense bodies, where it helps to anchor the myofibrillar actin filaments. It is a Homodimer and also forms heterodimers with ACTN2 and interacts with MYOZ1. Although about 18% of the world population lack a functional ACTN3 gnen due to a stop codon polymorphism at position 577, the absence of functional ACTN3 expression is not correlated with a disease state. Highly significant associations between ACTN3 genotype and athletic performance have been demonstrated in both male and female athletes.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product: Glycerol MSDS Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ACTN3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ACTN3 antibody images, references, products related to ab65067 and other tools.
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ACTN3 antibody images:
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Western blot - ACTN3 antibody (ab65067)
All lanes : ACTN3 antibody (ab65067) at 1/500 dilution
Lane 1 : extracts from HT-29 cells Lane 2 : extracts from HT-29 cells with immunizing peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 103 kDa Observed band size : 103 kDa
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Search PubMed (MEDLINE) for references to ACTN3
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ACTN3 antibody - more information
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Customer reviews (feedback) regarding ACTN3 antibody |
Customer FAQs regarding ACTN3 antibody |
Protocols for ACTN3 antibody |
Price and availability of products related to ACTN3 antibody |
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ACTN3 antibody - related products:
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ACTN3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ACTN3 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"