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Overview

  • Product nameACTR1A protein (Tagged-His Tag)See all ACTR1A proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 97-376 (280 amino acids) of Human ACTR1A with an N terminal His tag. Predicted MWt: 33 kDa UniProt ID: P61163.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 125 µl distilled water.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesDQLQTFSEEHPVLLTEAPLNPRKNRERAAEVFFETFNVPA LFISMQAVLSLY ATGRTTGVVLDSGDGVTHAVPIYEGF AMPHSIMRIDIAGRDVSRFLRLYLRK EGYDFHSSSEFE IVKAIKERACYLSINPQKDETLETEKAQYYLPDGSTIEIG PSRFRAPELLFRPDLIGEESEGIHEVLVFAIQKSDMDL RRTLFSNIVLSGGS TLFKGFGDRLLSEVKKLAPKDVKI RISAPQERLYSTWIGGSILASLDTFKKM WVSKKEYEED GARSIHRKTF
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91878 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    MS
    SDS-PAGE
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Actin related protein 1Actin RPVActin-RPV
        ACTR 1AActr1aACTZ_HUMANAlpha centractinAlpha-centractinARP 1ARP1ARP1 actin related protein 1 homolog AARP1 actin related protein 1 homolog A centractin alphaARP1 yeast homolog ACentractinCentractin alphaCentrosome associated actin homologCentrosome-associated actin homologCTRN 1CTRN1FLJ52695FLJ52800FLJ55002
      see all
  • FunctionComponent of a multi-subunit complex involved in microtubule based vesicle motility. It is associated with the centrosome.
  • Sequence similaritiesBelongs to the actin family. ARP1 subfamily.
  • Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome.

    • Target information above from: UniProt accession P61163 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    ACTR1A protein (Tagged-His Tag) images

    • SDS-PAGE - ACTR1A protein (Tagged-His Tag) (ab91878)
      SDS-PAGE - ACTR1A protein (Tagged-His Tag) (ab91878)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for ACTR1A protein (Tagged-His Tag) (ab91878)

    ab91878 has not yet been referenced specifically in any publications.

    Publishing research using ab91878 ? Please let us know so that we can cite the reference in this datasheet

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