ACTR1B protein (Tagged-His Tag) (ab91772)
This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
Our Abpromise guarantee covers the use of ab91772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- Actin related protein 1BActin-related protein 1BACTR 1B
- ACTR1BACTY_HUMANARP 1BARP1 actin related protein 1 homolog BARP1 actin related protein 1 homolog B centractin betaARP1 actin related protein 1 homolog B centractin beta (yeast)ARP1 actin related protein 1 yeast homolog B centractin betaARP1 yeast homolog BARP1BBeta centractinBeta-centractinCentractin betaCentractin beta (Yeast)CTRN 2CTRN2PC 3PC3
ACTR1B protein (Tagged-His Tag) images
The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.
References for ACTR1B protein (Tagged-His Tag) (ab91772)
ab91772 has not yet been referenced specifically in any publications.