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Overview

  • Product nameACTR1B protein (Tagged-His Tag)See all ACTR1B proteins and peptides ...
  • Protein descriptionRecombinant full length protein, corresponding to amino acids 1-376 of Human ACTR1B with N terminal His tag, 46kDa. UniProt ID = P42025.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 84 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesMESYDIIANQPVVIDNGSGVIKAGFAGDQIPKYCFPNYVG RPKHMRVMAGALEGD LFIGPKAEEHRGLLTIRYPMEHG VVRDWNDMERIWQYVYSKDQLQTFSEEHPVLL TEAPLN PSKNREKAAEVFFETFNVPALFISMQAVLSLYATGRTTGV VLDSGDGVT HAVPIYEGFAMPHSIMRVDIAGRDVSRYL RLLLRKEGVDFHTSAEFEVVRTIKER ACYLSINPQKDE ALETEKVQYTLPDGSTLDVGPARFRAPELLFQPDLVGDES EGL HEVVAFAIHKSDMDLRRTLFANIVLSGGSTLFKGF GDRLLSEVKKLAPKDIKIKI SAPQERLYSTWIGGSILA SLDTFKKMWVSKKEYEEDGSRAIHRKTF
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91772 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Actin related protein 1BActin-related protein 1BACTR 1B
        ACTR1BACTY_HUMANARP 1BARP1 actin related protein 1 homolog BARP1 actin related protein 1 homolog B centractin betaARP1 actin related protein 1 homolog B centractin beta (yeast)ARP1 actin related protein 1 yeast homolog B centractin betaARP1 yeast homolog BARP1BBeta centractinBeta-centractinCentractin betaCentractin beta (Yeast)CTRN 2CTRN2PC 3PC3
      see all
  • FunctionComponent of a multi-subunit complex involved in microtubule based vesicle motility. It is associated with the centrosome.
  • Sequence similaritiesBelongs to the actin family. ARP1 subfamily.
  • Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome.

    • Target information above from: UniProt accession P42025 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    ACTR1B protein (Tagged-His Tag) images

    • Western blot - ACTR1B protein (Tagged-His Tag) (ab91772)
      Western blot - ACTR1B protein (Tagged-His Tag) (ab91772)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for ACTR1B protein (Tagged-His Tag) (ab91772)

    ab91772 has not yet been referenced specifically in any publications.

    Publishing research using ab91772 ? Please let us know so that we can cite the reference in this datasheet

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