|
|
|
|
Western blot - ACVRL1 antibody (ab37807)

(enlarge)
|
|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ACVRL1 antibody (ab37807)

(enlarge)
|
|
|
|
|
Product Name
|
ACVRL1 antibody
|
| |
See all ACVRL1 antibodies (5)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to ACVRL1
|
|
Immunogen
|
A KLH conjugated synthetic peptide selected from the sequence of human ACVRL1 within aa 1-100 (N-terminal).
|
|
Reacts with
(species key)
|
Hu, Ms
|
|
Tested applications
(see key)
|
ELISA, IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions ELISA: 1/1000. IHC-P: 1/50 - 1/100. WB: 1/100 - 1/500. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Mouse heart tissue lysate and hepatocarcinoma tissue
|
|
Cellular localization
|
Membrane; single-pass type I membrane protein
|
|
Research areas
|
Cardiovascular >> Vasculature >> Endothelium Signal Transduction >> Growth Factors/Hormones >> TGF Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases Signal Transduction >> Metabolism >> Amino Acids Cardiovascular >> Angiogenesis >> Growth Factors >> TGF
|
|
Relevance
|
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation.
With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window
|
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Protein G purified
|
|
Storage buffer
|
Preservative: 0.09% Sodium Azide Constituents: PBS Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
|
|
Purification notes
|
This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
|
|
Form
|
Liquid
|
|
Concentration
|
0.250 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ACVRL1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for ACVRL1 antibody images, references, products related to ab37807 and other tools.
|
| |
ACVRL1 antibody images:
|
Western blot - ACVRL1 antibody (ab37807)
ACVRL1 antibody (ab37807) at 1/100 dilution + mouse heart tissue lysate
Predicted band size : 56 kDa Observed band size : 56 kDa Additional bands at : 25 kDa,45 kDa,70 kDa. We are unsure as to the identity of these extra bands.
|
| |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ACVRL1 antibody (ab37807)
ab37807 at a 1/50 dilution detecting ACVRL1 expression in human hepatocarcinoma tissue (a peroxidase-conjugated secondary antibody, followed by AEC staining was used).
These data demonstrate the suitability of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
|
| |
| |
|
Search PubMed (MEDLINE) for references to ACVRL1
|
| |
ACVRL1 antibody - more information
|
Customer reviews (feedback) regarding ACVRL1 antibody |
Customer FAQs regarding ACVRL1 antibody |
Protocols for ACVRL1 antibody |
Price and availability of products related to ACVRL1 antibody |
| |
ACVRL1 antibody - related products:
|
|
|
| |
ACVRL1 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about ACVRL1 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"