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Products:Cardiovascular >> Angiogenesis >> Growth Factors >> TGF
Overview
Product name
Anti-ACVRL1 antibody
See all ACVRL1 products (7) ...
Description
Rabbit polyclonal to ACVRL1
Tested applications
IHC-P, ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Non Human Primates
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human ACVRL1.
(Peptide available as ab86637.)
Positive control
This antibody gave a positive signal in the following whole cell lysates: K562, MCF7.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Vasculature >> Endothelium
Signal Transduction >> Growth Factors/Hormones >> TGF
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Signal Transduction >> Metabolism >> Amino Acids
Cardiovascular >> Angiogenesis >> Growth Factors >> TGF
Applications
Show applications key
Our Abpromise guarantee covers the use of ab68703 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 5 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF: Use a concentration of 5 µg/ml
WB: Use a concentration of 1 µg/mlDetects a band of approximately 62 kDa (predicted molecular weight: 56 kDa).
Target
Function
On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for TGF-beta. May bind activin as well.
Involvement in disease
Defects in ACVRL1 are the cause of hereditary hemorrhagic telangiectasia type 2 (HHT2) [MIM:600376]; also known as Osler-Rendu-Weber syndrome 2 (ORW2). HHT2 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary, cerebral and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia.
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
Contains 1 GS domain.
Contains 1 protein kinase domain.
Cellular localization
Membrane.
Target information above from: UniProt accessionP37023
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Activin A receptor antibody
- Activin A receptor type II like 1 antibody
- Activin receptor like kinase 1 antibody
see all
Database links
- Entrez Gene: 94 Human
- Omim: 601284 Human
- SwissProt: P37023 Human
- Unigene: 591026 Human
Anti-ACVRL1 antibody images:
Western blot - ACVRL1 antibody (ab68703)
All lanes : Anti-ACVRL1 antibody (ab68703) at 1 µg/ml
Lane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Predicted band size : 56 kDa
Observed band size : 62 kDa (why is the actual band size different from the predicted?)
ACVRL1 contains a number of potential glycosylation and phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Immunocytochemistry/ Immunofluorescence - ACVRL1 antibody (ab68703)
ICC/IF image of ab68703 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68703, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HepG2 and MCF7 cells at 5µg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ACVRL1 antibody (ab68703)
IHC image of ACVRL1 staining in normal human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68703, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
References for Anti-ACVRL1 antibody (ab68703)
ab68703 has not yet been referenced specifically in any publications.
Publishing research using ab68703? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"