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Products:Cardiovascular >> Angiogenesis >> Growth Factors >> TGF
Overview
Product name
Anti-ACVRL1 antibody
See all ACVRL1 products (7) ...
Description
Rabbit polyclonal to ACVRL1
Specificity
This antibody detects endogenous levels of total ACVRL1 protein.
Tested applications
WB, IHC-P, ICC/IF, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Rat
Immunogen
A synthesized peptide derived from the internal region of human ACVL1.
Positive control
Human breast carcinoma tissue sections, Hela cells and extracts from K562 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Vasculature >> Endothelium
Signal Transduction >> Growth Factors/Hormones >> TGF
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Signal Transduction >> Metabolism >> Amino Acids
Cardiovascular >> Angiogenesis >> Growth Factors >> TGF
Applications
Show applications key
Our Abpromise guarantee covers the use of ab74039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Predicted molecular weight: 56 kDa.
IHC-P: 1/50 - 1/100.
ICC/IF: 1/500 - 1/1000.
ELISA
ELISA: 1/20000ELISA: 1/20000
Target
Function
On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for TGF-beta. May bind activin as well.
Involvement in disease
Defects in ACVRL1 are the cause of hereditary hemorrhagic telangiectasia type 2 (HHT2) [MIM:600376]; also known as Osler-Rendu-Weber syndrome 2 (ORW2). HHT2 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary, cerebral and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia.
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
Contains 1 GS domain.
Contains 1 protein kinase domain.
Cellular localization
Membrane.
Target information above from: UniProt accessionP37023
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Activin A receptor antibody
- Activin A receptor type II like 1 antibody
- Activin receptor like kinase 1 antibody
see all
Database links
- Entrez Gene: 94 Human
- Entrez Gene: 25237 Rat
- Omim: 601284 Human
- SwissProt: P37023 Human
- SwissProt: P80203 Rat
- Unigene: 591026 Human
- Unigene: 10631 Rat
Anti-ACVRL1 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ACVRL1 antibody (ab74039)
Immunohistochemical analysis of ACVRL1 in paraffin embedded human breast carcinoma tissue using ab74039 at a 1/50 dilution.
Left image: untreated.
Right image: treated with immunizing peptide.
Western blot - ACVRL1 antibody (ab74039)
All lanes : Anti-ACVRL1 antibody (ab74039) at 1/500 dilution
Lane 1 : Extracts from K562 cells
Lane 2 : Extracts from K562 cells with immunizing peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 56 kDa
Observed band size : 56 kDa
Immunocytochemistry/ Immunofluorescence - ACVRL1 antibody (ab74039)
Immunofluorescence analysis of ACVRL1 in HeLa cells using ab74039 at a 1/500 dilution.
Left image: untreated.
Right image: treated with immunizing peptide.
References for Anti-ACVRL1 antibody (ab74039)
ab74039 has not yet been referenced specifically in any publications.
Publishing research using ab74039? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"