Anti-ADA antibody (Biotin) (ab34677)

Dear Sir / Madam,                            

Let us know if these ABCAM ADA antibodies(Cat no: Ab34677-ADA rabbit Pab& Ab54969-ADA mouse Mab) can be used as a pair for ELISA purpose or you are having other ADA matched antibody pair.                            

Also let us know if you can provide Ab54969-ADA mouse Mab at minimum 2-3mg concentration as for our R&D of ICT test this concentration is required.   If these two ADA antibodies(Cat no: Ab34677-ADA rabbit Pab& Ab54969-ADA mouse Mab) can be used as pair and if they can be available at minimum 2-3 mg concentration.    Kindly send the quotation for 5 mg bulk amount ADA antibodies each (Cat no: Ab34677-ADA rabbit Pab& Ab54969-ADA mouse Mab) at the best discounted prices.    Best Regards, Thanking you, Your Sincerely,    

ANSWER:

Thank you for your enquiry and your interest in our products.

According to the datasheets, ab54969 has not been tested for ELISA (only WB, Flow Cytmetry); whilst the other antibody (ab34677) has been characterized for indirect ELISA system. Unfortunately, these two antibodies have not been used as a pair in Sandwich ELISA (sELISA). I have conducted a search for you and though we have 8 antibodies against this target (ADA) in our catalogue, none have been tested specifically for sELISA.

Apologies! If you need anything further or any help then please let me know.  

Please send a western blot image that confirms the antibody detects a protein at the expected molecular weight of ADA, 40-41 kDa. Why does the datasheet list the predicted molecular weight as 104 kDa?

ANSWER:

Thank you for your patience while I investigated the source of the note on our datasheet that specified the molecular weight of ADA as 104 kDa.

The predicted molecular weight should be given as 40.7 kDa, based on the UniProt database entry:

http://www.uniprot.org/uniprot/P00813

The notation that specified the molecular weight as 104 kDa appears to have been a mistake and was not based on data.

What I found when investigating the source of the note was that the antibody had not in fact been tested in western blotting. The application has been removed from the datasheet. It is possible the antibody will stain a western blot effectively, but we do not currently guarantee this.

I apologize for any inconvenience. Although we do not have another biotinylated antibody against ADA, we have several unconjugated anti-ADA antibodies, including the rabbit monoclonal ab108352, which recognizes a protein at the predicted molecular weight for ADA.

http://www.abcam.com/index.html?datasheet=108352