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Product Name
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ADA antibody (Biotin)
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See all ADA antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ADA (Biotin)
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Immunogen
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Adenosine Deaminase from Calf spleen.
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Reacts with
(species key)
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Cow
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Specificity
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Purified and partially purified Adenosine Deaminase.
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Tested applications
(see key)
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Dot, ELISA, IM, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Dot: Use at an assay dependent dilution.
ELISA: 1/15000 - 1/64000.
This antibody has been assayed against 1.0µg of Adenosine Deaminase in a standard capture ELISA using Peroxidase conjugated streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at RT.
IM: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 104 kDa.
Suitable for other antibody based assays using streptavidin or avidin conjugates.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Deamination
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA / Nucleotides
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Relevance
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ADA (Adenosine deaminase) is an enzyme involved in the purine catabolic pathway. It irreversibly deaminates adenosine, converting it to the related nucleoside inosine by the removal of an amine group. Defects in ADA are a cause of autosomal recessive severe combined immuno-deficiency (SCID).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Conjugation notes
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10-20 Biotin molecules per Rabbit IgG molecule.
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Storage buffer
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Preservative: 0.01% (w/v) Sodium Azide
Constituents: 10mg/ml BSA (IgG and Protease free), 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against buffer.
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Form
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Liquid
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Conjugation
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Biotin
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Concentration
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10.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ADA antibody (Biotin) is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to ADA
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ADA antibody (Biotin) - more information
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Customer reviews (feedback) regarding ADA antibody (Biotin) |
Customer FAQs regarding ADA antibody (Biotin) |
Protocols for ADA antibody (Biotin) |
Price and availability of products related to ADA antibody (Biotin) |
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ADA antibody (Biotin) - related products:
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ADA antibody (Biotin) - other tools:
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Contact Abcam with a Technical Enquiry about ADA antibody (Biotin) |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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