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Product Name 

ADA antibody (HRP)

 

See all ADA antibodies (6)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ADA (HRP)

Immunogen 

Adenosine Deaminase (Calf spleen).

Reacts with 
(species key)

Cow

Specificity 

ab34651 recognises Adenosine Deaminase.

Tested applications 
(see key)

Dot, ELISA, EM, WB


Abreviews 

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Application notes 
(see key)

Recommended dilutions
Dot: Use at an assay dependent dilution.
Immunoperoxidase Electron Microscopy: Use at an assay dependent dilution.
ELISA: 1/20000 - 1/100000.
This antibody has been assayed against 1.0µg of Adenosine Deaminase in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at RT.
WB: Use at an assay dependent dilution. Predicted molecular weight: 104 kDa.
Suitable for other peroxidase-antibody based assays.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Deamination
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA / Nucleotides

Relevance 

ADA (Adenosine deaminase) is an enzyme involved in the purine catabolic pathway. It irreversibly deaminates adenosine, converting it to the related nucleoside inosine by the removal of an amine group. Defects in ADA are a cause of autosomal recessive severe combined immuno-deficiency (SCID).

 

ADA antibody (HRP) (ab34651)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

280712    (Cow)

SwissProt

    

P56658    (Cow)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

Preservative: 0.01% (w/v) Gentamicin Sulfate
Constituents: 10mg/ml BSA (IgG and Protease free), 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2

Purification notes 

This product is purified by a multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against buffer.

Form 

Liquid

Conjugation 

Horse Radish Peroxidase

Concentration 

10.000 mg/ml

Storage instructions 

Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ADA antibody (HRP) is on this datasheet. But please do contact us if you would like any reassurance!


 

Search PubMed (MEDLINE) for references to ADA

 

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Price and availability of products related to ADA antibody (HRP)

 

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Lysates

 

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Other ADA Antibodies

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ADA antibody (HRP) - other tools:

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