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Bonjour, |
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ANSWER: |
Merci pour votre émail et pour nous alerter de cette incohérence. |
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Dear Sir / Madam, Let us know if these ABCAM ADA antibodies(Cat no: Ab34677-ADA rabbit Pab& Ab54969-ADA mouse Mab) can be used as a pair for ELISA purpose or you are having other ADA matched antibody pair. Also let us know if you can provide Ab54969-ADA mouse Mab at minimum 2-3mg concentration as for our R&D of ICT test this concentration is required. If these two ADA antibodies(Cat no: Ab34677-ADA rabbit Pab& Ab54969-ADA mouse Mab) can be used as pair and if they can be available at minimum 2-3 mg concentration. Kindly send the quotation for 5 mg bulk amount ADA antibodies each (Cat no: Ab34677-ADA rabbit Pab& Ab54969-ADA mouse Mab) at the best discounted prices. Best Regards, Thanking you, Your Sincerely, |
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ANSWER: |
Thank you for your enquiry and your interest in our products. According to the datasheets, ab54969 has not been tested for ELISA (only WB, Flow Cytmetry); whilst the other antibody (ab34677) has been characterized for indirect ELISA system. Unfortunately, these two antibodies have not been used as a pair in Sandwich ELISA (sELISA). I have conducted a search for you and though we have 8 antibodies against this target (ADA) in our catalogue, none have been tested specifically for sELISA. Apologies! If you need anything further or any help then please let me know. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Overlay histogram showing Jurkat cells stained with ab54969 (red line). The cells were fixed with 80% methanol (5 min) and then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54969, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
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