Overview
Product nameADA protein (Active)See all ADA proteins and peptides ...
Protein descriptionRecombinant full length Human ADA (amino acids 1-363) with an N terminal His tag; 383aa, 42.9kDa inclusive of tag. (NP_000013)
Uniprot accessionP00813
Molecular weight42.900kDa
Protein length363 amino acids
Expression hostE. coli
Properties
Purity> 85
% by SDS-PAGE
Purification notesNo detergents such as urea, Triton or Tween were used to purify the proteins. Purified by using anion exchange chromatography (DEAE sepharose resin) and gel filtration chromatography (Sephacryl S 200) with 20mM Tris pH 7.5, 2mM EDTA.
Biological activityBiological activity: Specific activity: approximately >25 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme that deaminates 1.0 umol of adenosine to inosine per minute at pH 7.5 at 25oC
Mass spectrometryMALDI-TOF
FormLiquid
Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
Storage bufferpH: 8.00
Constituents: 0.32% Tris HCl, 20% Glycerol, 0.02% DTT
Concentration information loading...
SequenceMGSSHHHHHH SSGLVPRGSH MAQTPAFDKP KVELHVHLDG SIKPETILYY GRRRGIALPA NTAEGLLNVI GMDKPLTLPD FLAKFDYYMP AIAGCREAIK RIAYEFVEMK AKEGVVYVEV RYSPHLLANS KVEPIPWNQA EGDLTPDEVV ALVGQGLQEG ERDFGVKARS ILCCMRHQPN WSPKVVELCK KYQQQTVVAI DLAGDETIPG SSLLPGHVQA YQEAVKSGIH RTVHAGEVGS AEVVKEAVDI LKTERLGHGY HTLEDQALYN RLRQENMHFE ICPWSSYLTG AWKPDTEHAV IRLKNDQANY SLNTDDPLIF KSTLDTDYQM TKRDMGFTEE EFKRLNINAA KSSFLPEDEK RELLDLLYKA YGMPPSASAG QNL
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab109839
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| Functional Studies |
FuncS: Use at an assay dependent dilution. 1. Prerapre a 1.5 ml reaction mix: the final concentrations are 53.3mM potassium phosphate, 0.045mM adenosine, 0.003% (w/v) bovine serum.
2. Add ab109839 with various concentrations (0.1ug, 0.2) in assay buffer.
3. Mix by inversion and record A260nm for approximately 5 minutes.
|
| SDS-PAGE |
SDS-PAGE: Use at an assay dependent dilution. |
| MS |
MS: Use at an assay dependent dilution. |
Application notesThis peptide can be used with studies using ab170526.
Protein info
-
Alternative names
adaADA_HUMANAdenosine aminohydrolaseAdenosine deaminase
FunctionCatalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion.
Tissue specificityFound in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues.
Involvement in diseaseDefects in ADA are the cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-negative due to adenosine deaminase deficiency (ADASCID) [MIM:102700]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. ADA-SCID is an autosomal recessive form accounting for about 50% of non-X-linked SCIDs. ADA deficiency has been diagnosed in chronically ill teenagers and adults (late or adult onset). Population and newborn screening programs have also identified several healthy individuals with normal immunity who have partial ADA deficiency.
Sequence similaritiesBelongs to the adenosine and AMP deaminases family.
Cellular localizationCell membrane. Cell junction. Cytoplasmic vesicle lumen. Cytoplasm. Colocalized with DPP4 at the cell junction in lymphocyte-epithelial cell adhesion.
ADA protein (Active) images
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
SDS-PAGE - ADA protein (Active) (ab109839)SDS-PAGE analysis of ab109839: (1) MW marker, (2) ab109839 at 3µg