ADA protein (Active) (ab109839)
Constituents: 0.32% Tris HCl, 20% Glycerol, 0.02% DTT
Our Abpromise guarantee covers the use of ab109839 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||FuncS: Use at an assay dependent dilution. 1. Prerapre a 1.5 ml reaction mix: the final concentrations are 53.3mM potassium phosphate, 0.045mM adenosine, 0.003% (w/v) bovine serum. 2. Add ab109839 with various concentrations (0.1ug, 0.2) in assay buffer. 3. Mix by inversion and record A260nm for approximately 5 minutes.|
|SDS-PAGE||SDS-PAGE: Use at an assay dependent dilution.|
|MS||MS: Use at an assay dependent dilution.|
- adaADA_HUMANAdenosine aminohydrolaseAdenosine deaminase
ADA protein (Active) images
SDS-PAGE analysis of ab109839: (1) MW marker, (2) ab109839 at 3µg
Gel concentration: 15%
References for ADA protein (Active) (ab109839)
This product has been referenced in:
- Ginés S et al. Regulation of epithelial and lymphocyte cell adhesion by adenosine deaminase-CD26 interaction. Biochem J 361:203-9 (2002). Read more (PubMed: 11772392) »
- Daddona PE et al. Human adenosine deaminase. cDNA and complete primary amino acid sequence. J Biol Chem 259:12101-6 (1984). Read more (PubMed: 6090454) »