Anti-ADA2 antibody (ab215524)
Key features and details
- Rabbit polyclonal to ADA2
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
Overview
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Product name
Anti-ADA2 antibody
See all ADA2 primary antibodies -
Description
Rabbit polyclonal to ADA2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Recombinant full length protein (His-tag) corresponding to Saccharomyces cerevisiae ADA2 aa 1 to the C-terminus. Recombinant protein produced in E. coli.
Database link: Q02336 -
Positive control
- WB; whole cell extract of S. cerevisiae.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Preservative: 0.1% Sodium azide -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab215524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000.
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Notes |
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WB
1/500 - 1/1000. |
Target
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Relevance
Functions as component of the transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA and ADA. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA, an altered form of SAGA, may be involved in positive transcriptional regulation. SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus. ADA preferentially acetylates nucleosomal histones H3 (to form H3K14ac and H3K18ac) and H2B. -
Database links
- Entrez Gene: 852059 Saccharomyces cerevisiae
- SwissProt: Q02336 Saccharomyces cerevisiae
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Alternative names
- D9461.33 antibody
- SWI8 antibody
- Transcriptional adapter 2 antibody
- YDR448W antibody
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab215524 has been referenced in 1 publication.
- Camponeschi I et al. Light-Stress Response Mediated by the Transcription Factor KlMga2 in the Yeast Kluyveromyces lactis. Front Microbiol 12:705012 (2021). PubMed: 34335537