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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Overview
Product name
Anti-ADAM1 antibody
See all ADAM1 products (4) ...
Description
Rabbit polyclonal to ADAM1
Specificity
ab65878 recognizes ADAM1 alpha, but there is low homology with ADAM1 beta at the epitope.
Tested applications
Cross reactivity
Reacts with
Mouse
Immunogen
A synthetic peptide based on the EGF-like domain of mouse ADAM1.
Positive control
Mouse Epididymis and Testicle Lysates.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
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Western blot - ADAM1 antibody (ab65878)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab65878 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Detects a band of approximately 95 kDa (predicted molecular weight: 87 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
ADAM-1, also known as PH-30 alpha and Fertilin-alpha, was first described as a sperm-egg fusion protein from guinea pig. A member of the metalloproteinase family containing disintigrin-like domains (ADAMs), the function of ADAM-1 is still poorly understood. ADAM-1 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active. Like the other ADAMs, ADAM1 domain structure consists of a signal sequence followed by a propeptide domain, a metalloproteinase domain, a disintegrin domain cysteine-rich domain, an EGF-like domain, a transmembrane domain, and a cytoplasmic domain. ADAM1 is produced as two isoforms in the mouse, ADAM1 alpha and ADAM1 beta, which share 77% identity overall. The two sequences differ at the amino and carboxy termini, but are identical from residue 63 of ADAM1 alpha to residue 456, in the disintegrin domain. Both sequences are coded from intronless genes. Human ADAM1 is thought to be a pseudogene of the ADAM1 alpha sequence, and there is no report of a human homologue to mouse ADAM1 beta. The human gene has a stop codon and numerous insertions that change the reading frame of the gene, as well as a 26 bp deletion relative to the murine gene. It is interesting to note that abundant message for human ADAM1 is found in the testis, although there is report of protein produced. The mouse ADAM1 alpha message encodes a protein of 791 amino acids, with a predicted mass of 87.5 kDa and a pI of 5.2. The protein is reported at 100 kDa on SDS PAGE gels, and is processed to a 48 kDa form. ADAM1 beta message encodes a 806 amino acid protein, with predicted mass of 89.4 kDa and pI of4.66, seen as a 120 kDa precursor, processed to a 63-60 kDa form. Localized on the surface of sperm, the ADAM1 isoforms are thought to form a heterodimer with ADAM2 (fertilin-ß), and facilitate sperm-egg fusion, although there is some controversy about the precise actions the proteins play.
Cellular localization
Membrane; single-pass type I membrane protein
Alternative names
- A disintegrin and metalloproteinase domain 1a antibody
- Fertilin alpha 1a subunit antibody
- Ftna antibody
see all
Database links
- Entrez Gene: 280667 Mouse
- Entrez Gene: 280668 Mouse
- SwissProt: Q60813 Mouse
- SwissProt: Q8R534 Mouse
- Unigene: 1453 Mouse
Anti-ADAM1 antibody images:
Western blot - ADAM1 antibody (ab65878)
All lanes : Anti-ADAM1 antibody (ab65878) at 1/5000 dilution
Lane 1 : mouse epididymis lysate at 15 µl
Lane 2 : mouse epididymis lysate at 10 µl
Lane 3 : mouse epididymis lysate at 5 µl
Lane 4 : mouse testicle lysate at 15 µl
Lane 5 : mouse testicle lysate at 10 µl
Lane 6 : mouse testicle lysate at 5 µl
Predicted band size : 87 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa,36 kDa,45 kDa (possible cleavage fragment),55 kDa,66 kDa. We are unsure as to the identity of these extra bands.
ADAM1 alpha is reported to run at 100 kDa on SDS PAGE gels and is processed to a 48 kDa form.
References for Anti-ADAM1 antibody (ab65878)
ab65878 has not yet been referenced specifically in any publications.
Publishing research using ab65878? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"