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Anti-ADAM10 antibody - Aminoterminal end (ab39153)

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If your product does not perform as described on this datasheet, we will refund or replace your product...

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This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab39153 for help.

Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.

2 questions for ab39153

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Question 1

Wednesday 28-February-2007

I am expressing a series of constructs based on ADAM10 all beginning at amino acid 214 (TTSAEKN...) which is immediately downstream of the furin cleavage site. I'd like it confirmed that I will be able to pick up these derivatives with ab39153. Thank you

ANSWER:

  

Thank you for your enquiry and your interest in our products.

As I have mentioned in the previous mail, the information you requested is confidential and we have no access to you. However, after check with our source, I can confirm that the epitope for ab39153 begins past amino acid 214.

I hope this information will be useful for you.

Question 2

Tuesday 27-February-2007

The storage buffer contains 50% glycerol and 0.05% sodium azide per data sheet. Please confirm whether the buffer is "0.01 M phosphate buffered saline" per our listing.

Also, please ask whether vendor has assayed the purity by SDS and whether they have a spec for purity/.

ANSWER:

  

Thank you for your enquiry. The antibodies are >95% pure by SDS-PAGE. The sodium chloride concentration is 0.25 M. The phosphate concentration has changed slightly over the course of years as the peptide-affinity column elution protocol has been optimized, but it has always fallen within the range of 4 to 10 mM before adding glycerol to 50%, and is therefore 2 to 5 mM after the addition of glycerol.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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