ADAM10 antibody (ab10956)

Product Name 

ADAM10 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ADAM10

Immunogen 

Synthetic peptide corresponding to the amino terminal end of active ADAM10 (Human).

Reacts with 
(species key)

Hu

Specificity 

ab10956 recognises both the latent and active forms of ADAM10.

Tested applications 
(see key)

ELISA, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
WB: 1/1000. Predicted molecular weight: 89.8 kDa. EDTA/EGTA treatment of tissues or lysates is required to detect latent zymogen.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

ADAM10 is expressed in a wide range of tissues including Lung, heart, brain and kidney.

Cellular localization 

Cell membrane, Type I membrane protein. Also localized in the Golgi apparatus.

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Amyloid
Neuroscience >> Neurology process >> Notch Pathway
Cell Biology >> Apoptosis >> Receptors >> Receptor Processing

Relevance 

ADAM10 is a member of the ADAM (a disintegrin and metalloprotease like domain) family. It cleaves the membrane-bound precursor of TNF-alpha at 76-Ala-|-Val-77 to its mature soluble form and is responsible for the proteolytic release of several cell-surface proteins, including heparin binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). It contributes to the normal cleavage of the cellular prion protein and is involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicules, suggesting a vesicle-based protease activity.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, Whole Serum

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

Purified IgG was prepared from whole serum by affinity chromatography.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

This product should be stored undiluted.
Storage in frost-free freezers is not recommended.
Should this product contain a precipitate we recommend microcentrifugation before use.

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