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ab1997 has been referenced in 8 publications.
Publishing research using ab1997? Please let us know so that we can cite the reference in this datasheet
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All lanes : Anti-ADAM10 antibody (ab1997) at 1/1666 dilution
Lane 2 : ADAM10 siRNA treated human keratinocyte cells
Lane 3 : Non-treated human keratinocyte cells.
Secondary
HRP conjugated Goat anti-rabbit antibody
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 84 kDa
Observed band size : 75,100 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
This image is courtesy of an anonymous Abreview
All lanes : Anti-ADAM10 antibody (ab1997) at 1/2000 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : Jurkat whole cell lysate in presence of Blocking peptide at 1 µg/ml
Predicted band size : 84 kDa
Observed band size : 85 kDa (why is the actual band size different from the predicted?)
ICC/IF image of ab1997 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1997, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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