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Anti-ADAM10 antibody (ab1997)

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2 questions for ab1997

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Question 1

Thursday 26-July-2007

We purchased the antibodies ab1997 and ab39178 for my experiments. I can detect bands for the mouse AD10 proteins (wt or mutants I made) but I have problems interpreting my data with ab39178. I need to know with region of the propeptide was used as immunogen, in order to build hypotheses on the "what's going on with these mutants?". Due to the mutations, I theoretically have a different maturation than for wt-AD10 but I couldn't see any differences in the WB [my constructs are fully sequenced].

ANSWER:

  

I'm afraid I am unable to provide the exact immunogen sequence for this antibody as it is not made by Abcam and the supplier, for commercial reasons, cannot release it to us. I can tell you that the sequence is in the middle of the propeptide sequence, within the range of amino acids 80 - 120.

I'm sorry I cannot help you more on this occasion.

Question 2

Monday 22-October-2001

re ab1997 polyclonal rabbit anti ADAM 10: does this antibody a) x react with bovine ADAM 10 , I know it says the sequence is the same but just wanna check and b) does it work for immunocytochemistry on either frozen or wax sections?

Thanks

ANSWER:

  

Although this antibody has not been tested with bovine ADAM10, we predict that it will cross react as the AA sequence of the bovine peptide is 100% identical to AAs 732 to 748 of the human peptide used as the immunogen. To our knowledge, this product has not been tested in immunocytochemistry.

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