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Immunohistochemistry - ADAM10 antibody (ab1997)
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Western blot - ADAM10 antibody (ab1997)
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Western blot - ADAM10 antibody (ab1997)
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Product Name
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ADAM10 antibody
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See all ADAM10 antibodies (9)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ADAM10
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Immunogen
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Synthetic peptide, corresponding to amino acids 732-748 of Human ADAM10. This sequence is identical to those of bovine and rat origins and differs from that of mouse ADAM10 by one amino acid.
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Reacts with
(species key)
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Hu
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Epitope
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PQRQRPRESYQMGHMRR
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Tested applications
(see key)
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ELISA, ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
ICC/IF: Use at a concentration of 2 µg/ml.
IHC-P: 1/500.
WB: 1/500 - 1/2000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Jurkat whole cell lysate
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Cellular localization
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Cell membrane, Type I membrane protein. Also localized in the Golgi apparatus.
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Cell Biology >> Apoptosis >> Receptors >> Receptor Processing
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Relevance
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ADAM10 is a member of the ADAM (a disintegrin and metalloprotease like domain) family. It cleaves the membrane-bound precursor of TNF-alpha at 76-Ala-|-Val-77 to its mature soluble form and is responsible for the proteolytic release of several cell-surface proteins, including heparin binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). It contributes to the normal cleavage of the cellular prion protein and is involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicules, suggesting a vesicle-based protease activity.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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PBS with 0.02% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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TNFa converting enzyme.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ADAM10 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ADAM10 antibody images, references, products related to ab1997 and other tools.
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ADAM10 antibody images:
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 Immunohistochemistry - ADAM10 antibody (ab1997)
ab1997 at 2µg/ml staining K562 cells by ICC/IF.
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Western blot - ADAM10 antibody (ab1997)
All lanes : ADAM10 antibody (ab1997) at 1/1666 dilution
Lane 2 : ADAM10 siRNA treated human keratinocyte cells
Lane 3 : Non-treated human keratinocyte cells.
Secondary
HRP conjugated Goat anti-rabbit antibody
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 75,100 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute This image is courtesy of an anonymous Abreview
See Abreview
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Western blot - ADAM10 antibody (ab1997)
All lanes : ADAM10 antibody (ab1997) at 1/2000 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : Jurkat whole cell lysate in presence of Blocking peptide at 1 µg/ml
Observed band size : 85 kDa (why is the actual band size different from the predicted?)
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Immunocytochemistry/ Immunofluorescence-ADAM10 antibody(ab1997)
ICC/IF image of ab1997 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1997, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for ADAM10 antibody (ab1997)
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This product has been used in: (two most recent references)
Amadio M et al. nELAV proteins alteration in Alzheimer's disease brain: a novel putative target for amyloid-beta reverberating on AbetaPP processing. J Alzheimers Dis 16:409-19 (2009). WB; Human. PubMed: 19221430
Xu D et al. Tetraspanin12 regulates ADAM10-dependent cleavage of amyloid precursor protein. FASEB J 23:3674-81 (2009). PubMed: 19587294
See all 4 publication references for this product.
If you publish research using ab1997 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to ADAM10
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ADAM10 antibody - more information
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ADAM10 antibody - related products:
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ADAM10 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ADAM10 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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