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Products:Cell Biology >> Apoptosis >> Receptors >> Receptor Processing
Overview
Product name
Anti-ADAM10 antibody
See all ADAM10 products (10) ...
Description
Rabbit polyclonal to ADAM10
Tested applications
IHC-P, WB, ELISA, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide, corresponding to amino acids 732-748 of Human ADAM10. This sequence is identical to those of bovine and rat origins and differs from that of mouse ADAM10 by one amino acid.
Epitope
PQRQRPRESYQMGHMRR
Positive control
Jurkat whole cell lysate
General notes
TNFa converting enzyme.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.02% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Cell Biology >> Apoptosis >> Receptors >> Receptor Processing
Applications
Show applications key
Our Abpromise guarantee covers the use of ab1997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/500
WB: 1/500 - 1/2000.Predicted molecular weight: 84 kDa.
ELISA: Use at an assay dependent dilution.
ICC/IF: Use a concentration of 2 µg/ml
Target
Function
Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-
-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis.
Tissue specificity
Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
The precursor is cleaved by a furin endopeptidase.
Cellular localization
Cell membrane. Endomembrane system. Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi.
Target information above from: UniProt accessionO14672
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A disintegrin and metalloprotease domain 10 antibody
- A Disintegrin And Metalloproteinase Domain 10 antibody
- AD 10 antibody
see all
Database links
- Entrez Gene: 102 Human
- Entrez Gene: 11487 Mouse
- Omim: 602192 Human
- SwissProt: O14672 Human
- SwissProt: O35598 Mouse
- Unigene: 172028 Human
- Unigene: 578508 Human
- Unigene: 3037 Mouse
Anti-ADAM10 antibody images:
Western blot - ADAM10 antibody (ab1997)
All lanes : Anti-ADAM10 antibody (ab1997) at 1/1666 dilution
Lane 2 : ADAM10 siRNA treated human keratinocyte cells
Lane 3 : Non-treated human keratinocyte cells.
Secondary
HRP conjugated Goat anti-rabbit antibody
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 84 kDa
Observed band size : 75,100 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
This image is courtesy of an anonymous Abreview
Western blot - ADAM10 antibody (ab1997)
All lanes : Anti-ADAM10 antibody (ab1997) at 1/2000 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : Jurkat whole cell lysate in presence of Blocking peptide at 1 µg/ml
Predicted band size : 84 kDa
Observed band size : 85 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence-ADAM10 antibody(ab1997)
ICC/IF image of ab1997 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1997, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-ADAM10 antibody (ab1997)
This product has been referenced in:
- Doberstein Ket al. The transcription factor PAX2 regulates ADAM10 expression in renal cell carcinoma. Carcinogenesis : (2011).Read more (PubMed: 21880579) »
- Rabquer BJet al. Junctional Adhesion Molecule-C Is a Soluble Mediator of Angiogenesis. J Immunol : (2010). WB; Human.Read more (PubMed: 20592283) »
See all 8 publications for this product
Publishing research using ab1997? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"