Anti-ADAM12 antibody - Aminoterminal end (ab39155)
Overview
- Product nameAnti-ADAM12 antibody - Aminoterminal endSee all ADAM12 primary antibodies ...
- DescriptionRabbit polyclonal to ADAM12 - Aminoterminal end
- SpecificityThis antibody recognizes latent unprocessed and processed forms of ADAM12, but does not react with other ADAMs.
- Tested applicationsFlow Cyt, ICC/IF, WB more details
- Species reactivityReacts with: Mouse, Rat, Human, Pig
- Immunogen
Synthetic peptide based on the aminoterminal end of human ADAM12. (Peptide available as ab41204.)
- Positive controlCell lysate from human fetal fibroblasts (treated with TNF alpha).
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS containing 500mM Sodium chloride, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesThe antibody has been peptide affinity purified.
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab39155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Flow Cyt | Flow Cyt: Use a concentration of 2 µg/ml. PubMed: 19213876 |
| ICC/IF | ICC/IF: Use at an assay dependent dilution. |
| WB | WB: 1/1000 - 1/5000. Predicted molecular weight: 100 kDa.Can be blocked with ADAM12 peptide (Aminoterminal end) (ab41204). A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. |
Target
- FunctionInvolved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.
- Tissue specificityIsoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas.
- Sequence similaritiesContains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain. - DomainThe cysteine-rich domain supports cell adhesion through syndecans and triggers signaling events that lead to beta-1 integrin-dependent cell spreading. In carcinomas cells the binding of this domain to syndecans does not allow the integrin-mediated cell spreading.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. - Post-translational
modificationsThe precursor is cleaved by a furin endopeptidase. - Cellular localizationSecreted and Cell membrane.
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Database links
- Entrez Gene: 8038 Human
- Entrez Gene: 11489 Mouse
- Entrez Gene: 679837 Rat
- Omim: 602714 Human
- SwissProt: O43184 Human
- SwissProt: Q61824 Mouse
- Unigene: 594537 Human
- Unigene: 439714 Mouse
Target information above from: UniProt accession
O43184
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- A disintegrin and metalloproteinase domain 12 antibodyA disintegrin and metalloproteinase domain 12 antibodyADA12_HUMAN antibody
- ADAM 12 antibodyADAM metallopeptidase domain 12 antibodyADAM12 antibodyDisintegrin and metalloproteinase domain-containing protein 12 antibodyMCMP antibodyMCMPMltna antibodyMCMPMltna antibodyMeltrin alpha antibodyMeltrin-alpha antibodyMLTN antibodyMLTNA antibodyOTTHUMP00000046766 antibody
see all
Anti-ADAM12 antibody - Aminoterminal end images
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Western blot - ADAM12 antibody - Aminoterminal end (ab39155)Anti-ADAM12 antibody - Aminoterminal end (ab39155) at 1/1000 dilution +Heart (Human) Tissue Lysate - adult normal tissue (ab29431) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Predicted band size : 100 kDa
Observed band size : 84 kDa (why is the actual band size different from the predicted?)
Additional bands at : 117 kDa,42 kDa,50 kDa. We are unsure as to the identity of these extra bands.
Protocols
References for Anti-ADAM12 antibody - Aminoterminal end (ab39155)
This product has been referenced in:
- Estrella C et al. Role of A disintegrin and metalloprotease-12 in neutrophil recruitment induced by airway epithelium. Am J Respir Cell Mol Biol 41:449-58 (2009). Flow Cyt ; Human . Read more (PubMed: 19213876) »
