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Synthetic peptide based on the aminoterminal end of human ADAM12. (Peptide available as ab41204.)
Our Abpromise guarantee covers the use of ab39155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use a concentration of 2 µg/ml. PubMed: 19213876ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 100 kDa.Can be blocked with ADAM12 peptide (ab41204). A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 23671573|
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