Anti-ADAM12 antibody - Aminoterminal end (ab39155)

Overview

  • Product nameAnti-ADAM12 antibody - Aminoterminal endSee all ADAM12 primary antibodies ...
  • Description
    Rabbit polyclonal to ADAM12 - Aminoterminal end
  • SpecificityThis antibody recognizes latent unprocessed and processed forms of ADAM12, but does not react with other ADAMs.
  • Tested applicationsFlow Cyt, ICC/IF, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Synthetic peptide based on the aminoterminal end of human ADAM12. (Peptide available as ab41204.)

  • Positive control
    • Cell lysate from human fetal fibroblasts (treated with TNF alpha).

Properties

Applications

Our Abpromise guarantee covers the use of ab39155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 2 µg/ml. PubMed: 19213876
ICC/IF Use at an assay dependent concentration.
WB 1/1000 - 1/5000. Predicted molecular weight: 100 kDa.Can be blocked with ADAM12 peptide (ab41204). A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.
IHC-Fr Use at an assay dependent concentration. PubMed: 23671573

Target

  • FunctionInvolved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.
  • Tissue specificityIsoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas.
  • Sequence similaritiesContains 1 disintegrin domain.
    Contains 1 EGF-like domain.
    Contains 1 peptidase M12B domain.
  • DomainThe cysteine-rich domain supports cell adhesion through syndecans and triggers signaling events that lead to beta-1 integrin-dependent cell spreading. In carcinomas cells the binding of this domain to syndecans does not allow the integrin-mediated cell spreading.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    The precursor is cleaved by a furin endopeptidase.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A disintegrin and metalloproteinase domain 12 antibody
    • A disintegrin and metalloproteinase domain 12 antibody
    • ADA12_HUMAN antibody
    • ADAM 12 antibody
    • ADAM metallopeptidase domain 12 antibody
    • ADAM12 antibody
    • Disintegrin and metalloproteinase domain-containing protein 12 antibody
    • MCMP antibody
    • MCMPMltna antibody
    • MCMPMltna antibody
    • Meltrin alpha antibody
    • Meltrin-alpha antibody
    • MLTN antibody
    • MLTNA antibody
    • OTTHUMP00000046766 antibody
    see all

Anti-ADAM12 antibody - Aminoterminal end images

  • Anti-ADAM12 antibody - Aminoterminal end (ab39155) at 1/1000 dilution + Heart (Human) Tissue Lysate - adult normal tissue (ab29431) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution

    Predicted band size : 100 kDa
    Observed band size : 84 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 117 kDa,42 kDa,50 kDa. We are unsure as to the identity of these extra bands.

References for Anti-ADAM12 antibody - Aminoterminal end (ab39155)

This product has been referenced in:
  • Przewozniak M  et al. Adhesion proteins--an impact on skeletal myoblast differentiation. PLoS One 8:e61760 (2013). WB, IHC-Fr, ICC/IF ; Mouse, Rat . Read more (PubMed: 23671573) »
  • Estrella C  et al. Role of A disintegrin and metalloprotease-12 in neutrophil recruitment induced by airway epithelium. Am J Respir Cell Mol Biol 41:449-58 (2009). Flow Cyt ; Human . Read more (PubMed: 19213876) »

See all 2 Publications for this product

Product Wall

On our datasheet for ab39155 we suggest using human fetal fibroblasts as a positive control. Otherwise other positive controls which can be used are: Hela cells, A431 cells or human chondrosarcoma.



In this situation I think it would be helpful to get some more detail describing the preparation of reagents and experimental procedures used for western blotting and flow. If I can I will offer any tips to optimize the experiments to hopeful...

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Vielen Dank für Ihre Email und für Ihr Interesse an unseren Produkten.
Unserem Wissen nach wurde ab39155 bisher noch nicht in IHC-Fr getestet und auch nicht in dieser Anwendung publiziert.
Falls Sie dies selbst testen möchten, kann ich Ihnen ...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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No problem, I will send the product ab56366.

Could you let me know the purchase order number of ab39155?

Thanks!

Thank you for your email.

Lab has confirmed that the immunogen for ab115203 and ab28747 is indeed corresponds to N-terminal end and is in signal peptide region, so these antibodies might not be suitable.

Regarding ab56366 this antibod...

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I have requested confirmation of immunogen sequence from lab. I will get back to you with the reply soon. Once it gets confirmed I will then be able to suggest the suitable antibody.

Many thanks for having patience.

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital ...

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Thank you for taking the time to provide us with the further details. I apologize for the delay of my reply. I am very sorry, that you are experiencing problems with two of our antibodies. I do actually suspect that you have no signal of Adam12 and the...

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Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (NIH3T3)
Specification NIH3T3
Fixative Paraformaldehyde
Permeabilization Yes - Triton-X-100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 22°C
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Submitted Dec 17 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"