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Products:Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
Overview
Product name
Anti-ADAM12 antibody - Aminoterminal end catalytic domain
See all ADAM12 products (9) ...
Description
Rabbit polyclonal to ADAM12 - Aminoterminal end catalytic domain
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat
Immunogen
Synthetic peptide based on the amino end of the catalytic domain of human ADAM 12.
(Peptide available as ab41205.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
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Western blot - ADAM12 antibody - Aminoterminal end catalytic domain (ab28225)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. (A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates.)
Target
Function
Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.
Tissue specificity
Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas.
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain.
Domain
The cysteine-rich domain supports cell adhesion through syndecans and triggers signaling events that lead to beta-1 integrin-dependent cell spreading. In carcinomas cells the binding of this domain to syndecans does not allow the integrin-mediated cell spreading.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
The precursor is cleaved by a furin endopeptidase.
Cellular localization
Secreted and Cell membrane.
Target information above from: UniProt accessionO43184
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A disintegrin and metalloproteinase domain 12 antibody
- A disintegrin and metalloproteinase domain 12 antibody
- ADA12_HUMAN antibody
see all
Database links
- Entrez Gene: 8038 Human
- Entrez Gene: 11489 Mouse
- Entrez Gene: 679837 Rat
- Omim: 602714 Human
- SwissProt: O43184 Human
- SwissProt: Q61824 Mouse
- Unigene: 594537 Human
- Unigene: 8850 Human
see all
Anti-ADAM12 antibody - Aminoterminal end catalytic domain images:
Western blot - ADAM12 antibody - Aminoterminal end catalytic domain (ab28225)
All lanes : Anti-ADAM12 antibody - Aminoterminal end catalytic domain (ab28225)
Lane 1 : Human LNCaP prostate cancer cells - whole cell lysate
Lane 2 : Human LNCaP prostate cancer cells - whole cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
HRP donkey anti-rabbit polyclonal antibody
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 68,92 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
This image is courtesy of an anonymous Abreview
Protocols
References for Anti-ADAM12 antibody - Aminoterminal end catalytic domain (ab28225)
This product has been referenced in:
- Zhang Let al. Adam12 plays a role during uterine decidualization in mice. Cell Tissue Res : (2009). WB; Mouse.Read more (PubMed: 19841944) »
- Estrella Cet al. Role of A disintegrin and metalloprotease-12 in neutrophil recruitment induced by airway epithelium. Am J Respir Cell Mol Biol 41:449-58 (2009). Flow Cyt; Human.Read more (PubMed: 19213876) »
See all 2 publications for this product
Publishing research using ab28225? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"