Overview

  • Product name
  • Description
    Goat polyclonal to ADAM12
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Human ADAM12 aa 225-239 (internal sequence) (Cysteine residue). (NP_003465.3).
    Sequence:

    C-REFQRQGKDLEKVKQ


    Database link: O43184

  • Positive control
    • WB: Human, mouse, rat and pig heart lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab223476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).

Target

  • Function
    Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.
  • Tissue specificity
    Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas.
  • Sequence similarities
    Contains 1 disintegrin domain.
    Contains 1 EGF-like domain.
    Contains 1 peptidase M12B domain.
  • Domain
    The cysteine-rich domain supports cell adhesion through syndecans and triggers signaling events that lead to beta-1 integrin-dependent cell spreading. In carcinomas cells the binding of this domain to syndecans does not allow the integrin-mediated cell spreading.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    The precursor is cleaved by a furin endopeptidase.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A disintegrin and metalloproteinase domain 12 antibody
    • ADA12_HUMAN antibody
    • ADAM 12 antibody
    • ADAM metallopeptidase domain 12 antibody
    • ADAM12 antibody
    • CAR10 antibody
    • Disintegrin and metalloproteinase domain-containing protein 12 antibody
    • MCMP antibody
    • MCMPMltna antibody
    • Meltrin alpha antibody
    • Meltrin-alpha antibody
    • Metalloprotease disintegrin 12 transmembrane antibody
    • MLTN antibody
    • MLTNA antibody
    • OTTHUMP00000046766 antibody
    see all

Images

  • Anti-ADAM12 antibody (ab223476) at 0.1 µg/ml + Human heart lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 100 kDa
    Observed band size : 100 kDa

    Primary incubation was 1 hour.

  • All lanes : Anti-ADAM12 antibody (ab223476) at 0.1 µg/ml

    Lane 1 : Mouse heart lysate (in RIPA buffer)
    Lane 2 : Rat heart lysate (in RIPA buffer)
    Lane 3 : Pig heart lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique

    Predicted band size : 100 kDa
    Observed band size : 60,90 kDa (why is the actual band size different from the predicted?)

    Primary incubation was 1 hour.

References

ab223476 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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