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Anti-ADAM12 antibody (ab28747) at 0.1 µg/ml + Human heart lysate (RIPA buffer, 30µg total protein per lane)
Predicted band size : 100 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
Primary incubation was for 1 hour.
Detected using chemiluminescence.
ab28747 at 3.8µg/ml staining ADAM12 in human prostate tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval using citrate pH 6 was performed. AP staining used as detection method.
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