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Products:Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
Overview
Product name
Anti-ADAM12 antibody
See all ADAM12 products (9) ...
Description
Goat polyclonal to ADAM12
Specificity
This antibody is expected to recognise both the longer membrane bound form of human ADAM12 and the shorter soluble ADAM12 splice isoform. This antibody does not cross react with other ADAMS.
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: AARPLPVSPARALC, corresponding to N terminal amino acids 2-15 of ADAM12.
AARPLPVSPA RALC
Positive control
Human heart lysate. Human prostate tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide.
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3.
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28747 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.1 - 0.3 µg/ml.Detects a band of approximately 95 kDa (predicted molecular weight: 100 kDa).
IHC-P: Use a concentration of 2 µg/mlPerform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.(PubMed: 19213876)
Target
Function
Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.
Tissue specificity
Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas.
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain.
Domain
The cysteine-rich domain supports cell adhesion through syndecans and triggers signaling events that lead to beta-1 integrin-dependent cell spreading. In carcinomas cells the binding of this domain to syndecans does not allow the integrin-mediated cell spreading.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
The precursor is cleaved by a furin endopeptidase.
Cellular localization
Secreted and Cell membrane.
Target information above from: UniProt accessionO43184
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A disintegrin and metalloproteinase domain 12 antibody
- ADA12_HUMAN antibody
- ADAM 12 antibody
see all
Database links
- Entrez Gene: 8038 Human
- Omim: 602714 Human
- SwissProt: O43184 Human
- Unigene: 594537 Human
- Unigene: 8850 Human
Anti-ADAM12 antibody images:
Western blot - ADAM12 antibody (ab28747)
Anti-ADAM12 antibody (ab28747) at 0.1 µg/ml + Human heart lysate (RIPA buffer, 30µg total protein per lane)
Predicted band size : 100 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
Primary incubation was for 1 hour.
Detected using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ADAM12 antibody (ab28747)
ab28747 at 3.8µg/ml staining ADAM12 in human prostate tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval using citrate pH 6 was performed. AP staining used as detection method.
References for Anti-ADAM12 antibody (ab28747)
This product has been referenced in:
- Estrella Cet al. Role of A disintegrin and metalloprotease-12 in neutrophil recruitment induced by airway epithelium. Am J Respir Cell Mol Biol 41:449-58 (2009). IHC-P; Human.Read more (PubMed: 19213876) »
See 1 publication for this product
Publishing research using ab28747? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"