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Western blot - ADAM12 antibody (ab28747)
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Product Name
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ADAM12 antibody
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See all ADAM12 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to ADAM12
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Immunogen
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Synthetic peptide: AARPLPVSPARALC, corresponding to N terminal amino acids 2-15 of ADAM12.
BLAST 'AARPLPVSPARALC' with  or 
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Reacts with
(species key)
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Hu
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Specificity
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This antibody is expected to recognise both the longer membrane bound form of human ADAM12 and the shorter soluble ADAM12 splice isoform.
This antibody does not cross react with other ADAMS.
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Tested applications
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/64000.
IHC-P: Use at a concentration of 2 µg/ml (PMID 19213876).
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 100 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
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Human heart lysate.
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Cellular localization
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Type I membrane protein (isoform 12L). A secreted form (isoform 12S) is produced by alternative splicing.
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
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Relevance
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ADAM12 is a member of the ADAM (a disintegrin and metalloprotease like domain) family. Two forms of ADAM12 have been described: ADAM12S and ADAM12L (short and long forms). The short form is a soluble form lacking the transmembrane and cytoplasmic domains. The short form of ADAM12 was reported to provoke myogenesis, and the lack of cytoplasmic domain suggests different regulation pathways (although both forms can be expressed in the same tissue). Other papers investigated the SH3 ligand domains in the cytoplasmic portion of ADAM12, demonstrating regulation routes for ADAM12 via Src and Src tyrosine kinase. ADAM12 is primarily expressed in muscle and bone (although placenta, osteoblasts, and many tumor cell lines also express ADAM12). ADAM12 supports cell adhesion by acting as a cell attachment molecule and binding integrins through the cysteine rich domain. A marker of skeletal muscle regeneration, it has been implicated in myoblast differentiation and fusion. ADAM12 contains the canonical HExxHxxxxxH zinc metalloproteinase motif. Full length ADAM12 (909 amino acids) has a predicted mass of 99.5 kDa, but due to glycosylation and cyteine rich regions, the reduced protein migrates to 114 kDa (unprocessed) and 84 kDa (processed). The soluble form of ADAM12 (738 amino acids, predicted mass of 80.5 kDa) is seen as a 92 kDa zymogen and 68 kDa furin processed form.
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ADAM12 antibody (ab28747) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide.
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3. Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ADAM12 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ADAM12 antibody images, references, products related to ab28747 and other tools.
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ADAM12 antibody images:
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 Western blot - ADAM12 antibody (ab28747)
ADAM12 antibody (ab28747) at 0.1 µg/ml + Human heart lysate (RIPA buffer, 30µg total protein per lane)
Predicted band size : 100 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
Primary incubation was for 1 hour.
Detected using chemiluminescence.
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References for ADAM12 antibody (ab28747)
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This product has been used in:
Estrella C et al. Role of A disintegrin and metalloprotease-12 in neutrophil recruitment induced by airway epithelium. Am J Respir Cell Mol Biol 41:449-58 (2009). IHC-P; Human. PubMed: 19213876
If you publish research using ab28747 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to ADAM12
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ADAM12 antibody - more information
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