Overview

  • Product nameAnti-ADAM15 antibody - Cytoplasmic domain
    See all ADAM15 primary antibodies
  • Description
    Rabbit polyclonal to ADAM15 - Cytoplasmic domain
  • Specificityab39159 recognises the cytoplasmic domain of ADAM15. This antibody recognizes zymogen and furin processed forms of ADAM15.
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide based on the cytoplasmic domain of human ADAM15.

    (Peptide available as ab41210.)

  • Positive control
    • Cell lysate from human chondrosarcoma (treated with retinoic acid).

Properties

Applications

Our Abpromise guarantee covers the use of ab39159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Recommended starting dilution of 1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Detects a band of approximately 90 kDa (predicted molecular weight: 88 kDa). Note: The antibody recognizes bands of 110 kDa and 90 kDa, the zymogen and furin processed forms of ADAM15. A series of cleaved products at 55 kDa and 40 kDa can also be seen in reduced Western blots of cell lysates. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionActive metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain.
    • Tissue specificityExpressed in colon and small intestine. Expressed in airway smooth muscle and glomerular mesangial cells (at protein level). Ubiquitously expressed. Overexpressed in atherosclerotic lesions. Constitutively expressed in cultured endothelium and smooth muscle. Expressed in chondrocytes. Expressed in airway smooth muscle and glomerular mesangial cells.
    • Sequence similaritiesContains 1 disintegrin domain.
      Contains 1 EGF-like domain.
      Contains 1 peptidase M12B domain.
    • DomainThe cytoplasmic domain interacts with endophilin I and sorting nexin 9.
      Disintegrin domain binds to integrin alphaV-beta3.
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Post-translational
      modifications
      The precursor is cleaved by a furin endopeptidase.
      Phosphorylation increases association with PTKs.
    • Cellular localizationEndomembrane system. Cell junction > adherens junction. Cell projection > cilium > flagellum. Cytoplasmic vesicle > secretory vesicle > acrosome. The majority of the protein is localized in a perinuclear compartment which may correspond to the trans-Golgi network or the late endosome. The pro-protein is the major detectable form on the cell surface, whereas the majority of the protein in the cell is processed.
    • Information by UniProt
    • Database links
    • Alternative names
      • A disintegrin and metalloproteinase domain 15 (metargidin) antibody
      • A disintegrin and metalloproteinase domain 15 antibody
      • A disintegrin and metalloproteinase domain 15 antibody
      • ADA15_HUMAN antibody
      • ADAM 15 antibody
      • ADAM metallopeptidase domain 15 antibody
      • Adam15 antibody
      • and cysteine-rich protein 15 antibody
      • Disintegrin and metalloproteinase domain-containing protein 15 antibody
      • disintegrin-like antibody
      • EC 3.4.24. antibody
      • MDC 15 antibody
      • MDC-15 antibody
      • MDC15 antibody
      • Metalloprotease RGD disintegrin protein antibody
      • Metalloproteinase like disintegrin like and cysteine rich protein 15 antibody
      • Metalloproteinase-like antibody
      • Metargidin antibody
      see all

    Anti-ADAM15 antibody - Cytoplasmic domain images

    • Anti-ADAM15 antibody - Cytoplasmic domain (ab39159) at 1/1000 dilution + cell media from human chondrosarcoma (treated with retinoic acid)

      Predicted band size : 88 kDa
      Observed band size : 84,90,110 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 55 kDa (possible cleavage fragment).

    References for Anti-ADAM15 antibody - Cytoplasmic domain (ab39159)

    This product has been referenced in:
    • Fried D  et al. ADAM15 protein amplifies focal adhesion kinase phosphorylation under genotoxic stress conditions. J Biol Chem 287:21214-23 (2012). WB . Read more (PubMed: 22544741) »

    See 1 Publication for this product

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