ADAM15 antibody - Cytoplasmic domain (ab39159)

Product Name 

ADAM15 antibody - Cytoplasmic domain

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ADAM15 - Cytoplasmic domain

Immunogen 

Synthetic peptide based on the cytoplasmic domain of human ADAM15.

(Peptide available as ab41210.)

Reacts with 
(species key)

Hu

Specificity 

ab39159 recognises the cytoplasmic domain of ADAM15. This antibody recognizes zymogen and furin processed forms of ADAM15.

We have a range of domain specific antibodies for this target. For a full list please see all ADAM15 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: Recommended starting dilution of 1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Detects a band of approximately 90 kDa (predicted molecular weight: 88 kDa). Note: The antibody recognizes bands of 110 kDa and 90 kDa, the zymogen and furin processed forms of ADAM15. A series of cleaved products at 55 kDa and 40 kDa can also be seen in reduced Western blots of cell lysates. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Cell lysate from human chondrosarcoma (treated with retinoic acid).

Cellular localization 

Membrane; single pass type I membrane protein.

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha

Relevance 

ADAM15, also known as Metargidin and MDC15, is a member of the ADAM (a disintegrin and metalloprotease like domain) family. It was first described in mammary epithelial carcinoma cells as a membrane anchored Metalloprotease Disintegrin with RGD sequence (MetaRGiDin). Mouse ADAM15 does not contain the RGD sequence; instead the mouse ADAM15 has a TDD in its place. Like ADAM9 and ADAM12, there are SH3 ligand domains in the cytoplasmic portion of ADAM15, suggesting regulation routes for ADAM15 via Src and Src tyrosine kinase. ADAM15 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active. Full length ADAM15, 814 amino acids, has a predicted mass of 85 kDa, but due to glycosylation and cysteine rich regions, the reduced protein migrates to 110 kDa (unprocessed) and 84 kDa (processed). ADAM15 is widely expressed in normal tissues and tumor cell lines, and is expressed on the cell surface, anchored by the transmembrane domain. It is also elevated in osteoarthritic and rheumatoid synovial tissues, suggesting an inflammatory role. ADAM15 may also act as a cell attachment molecule by binding the integrins avbeta3 and a5beta1 through the cysteine rich domain and/or RGD sequence.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

See below for ADAM15 antibody - Cytoplasmic domain images, references, products related to ab39159 and other tools.

ADAM15 antibody - Cytoplasmic domain images:

Customer reviews (feedback) regarding ADAM15 antibody - Cytoplasmic domain

Customer FAQs regarding ADAM15 antibody - Cytoplasmic domain

Protocols for ADAM15 antibody - Cytoplasmic domain

Price and availability of products related to ADAM15 antibody - Cytoplasmic domain

ADAM15 antibody - Cytoplasmic domain - related products: