Overview

  • Product name
    Anti-ADAM15 antibody - N-terminal
    See all ADAM15 primary antibodies
  • Description
    Rabbit polyclonal to ADAM15 - N-terminal
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within N terminal amino acids 1-255 of Human ADAM15 (Uniprot ID: Q13444).

  • Positive control
    • HeLa, HepG2, MOLT4 and NIH 3T3 whole cell lysates; Human skeletal muscle tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab137387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer (pH8.0)
WB 1/500 - 1/3000. Predicted molecular weight: 93 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 25333931

Target

  • Function
    Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain.
  • Tissue specificity
    Expressed in colon and small intestine. Expressed in airway smooth muscle and glomerular mesangial cells (at protein level). Ubiquitously expressed. Overexpressed in atherosclerotic lesions. Constitutively expressed in cultured endothelium and smooth muscle. Expressed in chondrocytes. Expressed in airway smooth muscle and glomerular mesangial cells.
  • Sequence similarities
    Contains 1 disintegrin domain.
    Contains 1 EGF-like domain.
    Contains 1 peptidase M12B domain.
  • Domain
    The cytoplasmic domain interacts with endophilin I and sorting nexin 9.
    Disintegrin domain binds to integrin alphaV-beta3.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    The precursor is cleaved by a furin endopeptidase.
    Phosphorylation increases association with PTKs.
  • Cellular localization
    Endomembrane system. Cell junction > adherens junction. Cell projection > cilium > flagellum. Cytoplasmic vesicle > secretory vesicle > acrosome. The majority of the protein is localized in a perinuclear compartment which may correspond to the trans-Golgi network or the late endosome. The pro-protein is the major detectable form on the cell surface, whereas the majority of the protein in the cell is processed.
  • Information by UniProt
  • Database links
  • Alternative names
    • A disintegrin and metalloproteinase domain 15 (metargidin) antibody
    • A disintegrin and metalloproteinase domain 15 antibody
    • ADA15_HUMAN antibody
    • ADAM 15 antibody
    • ADAM metallopeptidase domain 15 antibody
    • Adam15 antibody
    • and cysteine-rich protein 15 antibody
    • Disintegrin and metalloproteinase domain-containing protein 15 antibody
    • disintegrin-like antibody
    • EC 3.4.24. antibody
    • MDC 15 antibody
    • MDC-15 antibody
    • MDC15 antibody
    • Metalloprotease RGD disintegrin protein antibody
    • Metalloproteinase like disintegrin like and cysteine rich protein 15 antibody
    • Metalloproteinase-like antibody
    • Metargidin antibody
    see all

Images

  • Anti-ADAM15 antibody - N-terminal (ab137387) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 93 kDa
  • Anti-ADAM15 antibody - N-terminal (ab137387) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg

    Predicted band size : 93 kDa
  • Immunohistochemical analysis of paraffin-embedded Human skeletal muscle, labelling ADAM15 with ab137387 at 10µg/ml.

References

This product has been referenced in:
  • Chatterjee V  et al. MicroRNA-147b regulates vascular endothelial barrier function by targeting ADAM15 expression. PLoS One 9:e110286 (2014). WB, ICC/IF ; Human . Read more (PubMed: 25333931) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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