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Western blot - ADAM15 antibody (ab83883)

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Product Name
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ADAM15 antibody
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See all ADAM15 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ADAM15
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Immunogen
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Synthetic peptide corresponding to a region within the internal sequence amino acids 540-589 (QPAAPLCLQT ANTRGNAFGS CGRNPSGSYV SCTPRDAICG QLQCQTGRTQ) of human ADAM15 (NP_997079). BLAST 'QPAAPLCLQT ANTRGNAFGS CGRNPSGSYV SCTPRDAICG QLQCQTGRTQ ' with or 
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 93 kDa.
ELISA titre using peptide based assay, 1:62500.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human fetal brain lysate
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Cellular localization
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Membrane; single pass type I membrane protein.
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
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Relevance
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ADAM15, also known as Metargidin and MDC15, is a member of the ADAM (a disintegrin and metalloprotease like domain) family. It was first described in mammary epithelial carcinoma cells as a membrane anchored Metalloprotease Disintegrin with RGD sequence (MetaRGiDin). Mouse ADAM15 does not contain the RGD sequence; instead the mouse ADAM15 has a TDD in its place. Like ADAM9 and ADAM12, there are SH3 ligand domains in the cytoplasmic portion of ADAM15, suggesting regulation routes for ADAM15 via Src and Src tyrosine kinase. ADAM15 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active. Full length ADAM15, 814 amino acids, has a predicted mass of 85 kDa, but due to glycosylation and cysteine rich regions, the reduced protein migrates to 110 kDa (unprocessed) and 84 kDa (processed). ADAM15 is widely expressed in normal tissues and tumor cell lines, and is expressed on the cell surface, anchored by the transmembrane domain. It is also elevated in osteoarthritic and rheumatoid synovial tissues, suggesting an inflammatory role. ADAM15 may also act as a cell attachment molecule by binding the integrins avbeta3 and a5beta1 through the cysteine rich domain and/or RGD sequence.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None Constituents: 2% Sucrose, PBS
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Purification notes
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Purified by peptide affinity chromatography method.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ADAM15 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ADAM15 antibody images, references, products related to ab83883 and other tools.
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ADAM15 antibody images:
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Western blot - ADAM15 antibody (ab83883)
ADAM15 antibody (ab83883) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal brain lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 93 kDa Observed band size : 77 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to ADAM15
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ADAM15 antibody - more information
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