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Overview

  • Product nameADAM15 protein (Tagged)See all ADAM15 proteins and peptides ...
  • Protein descriptionRecombinant full length Human ADAM15 (amino acids 1-772) with N terminal proprietary tag; Predicted MWt 110.1 kDa inclusive of tag (NP_997074.1).
  • Uniprot accessionQ13444-10
  • Molecular weight110.100kDa inclusive of tags
  • Protein length772 amino acids
  • Expression hostWheat germ

    • Properties

  • E.C. Number3.4.24.-
  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferpH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl
  • Concentration information loading...
  • Additional notesProtein concentration is above or equal to 0.05 mg/ml.
    Best used within three months from the date of receipt.
  • Sequence notesMRLALLWALGLLGAGSPLPSWPLPNIGGTEEQQAESEKAP REPLEPQVLQDDLPISLKKVLQTSLPEPLRIKLELDGDSH ILELLQNRELVPGRPTLVWYQPDGTRVVSEGHTLENCCYQ GRVRGYAGSWVSICTCSGLRGLVVLTPERSYTLEQGPGDL QGPPIISRIQDLHLPGHTCALSWRESVHTQKPPEHPLGQR HIRRRRDVVTETKTVELVIVADHSEAQKYRDFQHLLNRTL EVALLLDTFFRPLNVRVALVGLEAWTQRDLVEISPNPAVT LENFLHWRRAHLLPRLPHDSAQLVTGTSFSGPTVGMAIQN SICSPDFSGGVNMDHSTSILGVASSIAHELGHSLGLDHDL PGNSCPCPGPAPAKTCIMEASTDFLPGLNFSNCSRRALEK ALLDGMGSCLFERLPSLPPMAAFCGNMFVEPGEQCDCGFL DDCVDPCCDSLTCQLRPGAQCASDGPCCQNCQLRPSGWQC RPTRGDCDLPEFCPGDSSQCPPDVSLGDGEPCAGGQAVCM HGRCASYAQQCQSLWGPGAQPAAPLCLQTANTRGNAFGSC GRNPSGSYVSCTPRDAICGQLQCQTGRTQPLLGSIRDLLW ETIDVNGTELNCSWVHLDLGSDVAQPLLTLPGTACGPGLV CIDHRCQRVDLLGAQECRSKCHGHGVCDSNRHCYCEEGWA PPDCTTQLKATSSLTTGLLLSLLVLLVLVMLGASYWYRAR LHQRLCQLKGPTCQYSLRGQPSPHPQGSHCLPTPRAGAHR VTCPAQGLESRP
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab131862 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent concentration.
    ELISA ELISA: Use at an assay dependent concentration.
    WB WB: Use at an assay dependent concentration.
  • Application notesThis peptide can be used with studies using ab137387.

    • Protein info

    • Alternative names
        A disintegrin and metalloproteinase domain 15ADA15_HUMANADAM 15
        ADAM metallopeptidase domain 15Adam15and cysteine-rich protein 15Disintegrin and metalloproteinase domain-containing protein 15disintegrin-likeEC 3.4.24.MDC 15MDC-15MDC15Metalloprotease RGD disintegrin proteinMetalloproteinase like disintegrin like and cysteine rich protein 15Metalloproteinase-likeMetargidin
      see all
  • FunctionActive metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain.
  • Tissue specificityExpressed in colon and small intestine. Expressed in airway smooth muscle and glomerular mesangial cells (at protein level). Ubiquitously expressed. Overexpressed in atherosclerotic lesions. Constitutively expressed in cultured endothelium and smooth muscle. Expressed in chondrocytes. Expressed in airway smooth muscle and glomerular mesangial cells.
  • Sequence similaritiesContains 1 disintegrin domain.
    Contains 1 EGF-like domain.
    Contains 1 peptidase M12B domain.
  • DomainThe cytoplasmic domain interacts with endophilin I and sorting nexin 9.
    Disintegrin domain binds to integrin alphaV-beta3.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications    The precursor is cleaved by a furin endopeptidase.
    Phosphorylation increases association with PTKs.
  • Cellular localizationEndomembrane system. Cell junction > adherens junction. Cell projection > cilium > flagellum. Cytoplasmic vesicle > secretory vesicle > acrosome. The majority of the protein is localized in a perinuclear compartment which may correspond to the trans-Golgi network or the late endosome. The pro-protein is the major detectable form on the cell surface, whereas the majority of the protein in the cell is processed.

    • Target information above from: UniProt accession Q13444 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    ADAM15 protein (Tagged) images

    • SDS-PAGE - ADAM15 protein (Tagged) (ab131862)
      SDS-PAGE - ADAM15 protein (Tagged) (ab131862)
      12.5% SDS-PAGE analysis of ab131862 stained with Coomassie Blue.

    References for ADAM15 protein (Tagged) (ab131862)

    ab131862 has not yet been referenced specifically in any publications.

    Publishing research using ab131862 ? Please let us know so that we can cite the reference in this datasheet

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