Anti-ADAM17 antibody - Activation site (ab39163)

Immunocytochemistry/ Immunofluorescence - ADAM17 antibody - Activation site (ab39163)

The image shows ab39163 detecting mouse ADAM17 expressed in transfected HeLa cells. The cells were fixed in methanol and incubated with 10 µg/ml ab39163 for 1 hour at 20°C.

Image taken from anonymous Abreview submitted in February 2008

Western blot - ADAM17 antibody - Activation site (ab39163)

Anti-ADAM17 antibody - Activation site (ab39163) at 1/4000 dilution + MDA-MB231 lysate

Secondary
Donkey anti-Rabbit at 1/10000 dilution

Predicted band size : 93 kDa

ab39163 detected ADAM17 protein in lysates of the highly invasive human breast cancer cell line MDA-MB231. ab39163 was incubated at a dilution of 1/4000 for 16 hours at 4°C in PBS + 5% Milk. For further details please refer to the Abreview.

Image taken from an anonymous Abreview submitted in September 2007

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ADAM17 antibody - Activation site (ab39163)

Ab39163 staining human normal colon tissue. Staining is localised to cellular membranes.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.