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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Overview
Product name
Anti-ADAM17 antibody - Activation site
See all ADAM17 products (14) ...
Description
Rabbit polyclonal to ADAM17 - Activation site
Specificity
This antibody recognizes ADAM17, but does not react with other ADAMs.
Tested applications
WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Pig
Immunogen
Synthetic peptide based on the activation site (cysteine switch and furin cleavage site) of human ADAM17.
(Peptide available as ab41215.)
Epitope
ab39163 reacts with an epitope located in the activation site (cysteine switch and furin cleavage site) of ADAM17.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs
Cancer >> Signal transduction >> Other
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Stem Cells >> Signaling Pathways >> Notch >> Secreted
Signal Transduction >> Adapters >> Transmembrane
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39163 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/5000.Detects a band of approximately 120 kDa (predicted molecular weight: 93 kDa).(1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Higher concentrations of antibody may be needed for samples from more distantly related species. Detects a band of approximately 120 kDa in reduced Western blots of conditioned media or cell lysates, which is converted to a 55-60 kDa band.Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.)
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF: Use at an assay dependent dilution.
Target
Function
Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway.
Tissue specificity
Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Domain
Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
The precursor is cleaved by a furin endopeptidase.
Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791.
Cellular localization
Membrane.
Target information above from: UniProt accessionP78536
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibody
- A disintegrin and metalloproteinase domain 17 antibody
- ADA17_HUMAN antibody
see all
Database links
- Entrez Gene: 6868 Human
- Entrez Gene: 11491 Mouse
- Entrez Gene: 57027 Rat
- Omim: 603639 Human
- SwissProt: P78536 Human
- SwissProt: Q9Z0F8 Mouse
- SwissProt: Q9Z1K9 Rat
- Unigene: 404914 Human
see all
Anti-ADAM17 antibody - Activation site images:
Immunocytochemistry/ Immunofluorescence - ADAM17 antibody - Activation site (ab39163)
The image shows ab39163 detecting mouse ADAM17 expressed in transfected HeLa cells. The cells were fixed in methanol and incubated with 10 µg/ml ab39163 for 1 hour at 20°C.
Image taken from anonymous Abreview submitted in February 2008
Western blot - ADAM17 antibody - Activation site (ab39163)
Anti-ADAM17 antibody - Activation site (ab39163) at 1/4000 dilution + MDA-MB231 lysate
Secondary
Donkey anti-Rabbit at 1/10000 dilution
Predicted band size : 93 kDa
ab39163 detected ADAM17 protein in lysates of the highly invasive human breast cancer cell line MDA-MB231. ab39163 was incubated at a dilution of 1/4000 for 16 hours at 4°C in PBS + 5% Milk. For further details please refer to the Abreview.
Image taken from an anonymous Abreview submitted in September 2007
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ADAM17 antibody - Activation site (ab39163)
Ab39163 staining human normal colon tissue. Staining is localised to cellular membranes.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Protocols
References for Anti-ADAM17 antibody - Activation site (ab39163)
This product has been referenced in:
- Takaguri Aet al. A disintegrin and metalloprotease 17 mediates neointimal hyperplasia in vasculature. Hypertension 57:841-5 (2011). IHC-P; Rat.Read more (PubMed: 21357274) »
- Restituito Set al. Synaptic autoregulation by metalloproteases and gamma-secretase. J Neurosci 31:12083-93 (2011). WB, ICC/IF.Read more (PubMed: 21865451) »
See all 6 publications for this product
Publishing research using ab39163? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"