|
|
|
|
Immunocytochemistry/ Immunofluorescence - ADAM17 antibody - Activation site (ab39163)
(enlarge)
|
|
Western blot - ADAM17 antibody - Activation site (ab39163)
(enlarge)
|
|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-ADAM17 antibody - Activation site(ab39163)
(enlarge)
|
|
|
|
|
Product Name
|
ADAM17 antibody - Activation site
|
| |
See all ADAM17 antibodies (12)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to ADAM17 - Activation site
|
|
Immunogen
|
Synthetic peptide based on the activation site (cysteine switch and furin cleavage site) of human ADAM17.
(Peptide available as ab41215.)
|
|
Reacts with
(species key)
|
Hu, Ms, Rat, Pig
|
|
Specificity
|
This antibody recognizes ADAM17, but does not react with other ADAMs.
We have a range of domain specific antibodies for this target. For a full list please see all ADAM17 antibodies
|
|
Epitope
|
ab39163 reacts with an epitope located in the activation site (cysteine switch and furin cleavage site) of ADAM17.
|
|
Tested applications
(see key)
|
ICC/IF, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ICC/IF: Use at an assay dependent concentration.
WB: 1/1000 - 1/5000. Detects a band of approximately 120 kDa (predicted molecular weight: 93 kDa). WB: Recommended starting dilution of 1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Higher concentrations of antibody may be needed for samples from more distantly related species. Detects a band of approximately 120 kDa in reduced Western blots of conditioned media or cell lysates, which is converted to a 55-60 kDa band. (Predicted molecular weight: 93 kDa). Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Membrane; single pass type I membrane protein.
|
|
Research areas
|
Cancer >> Cancer Metabolism >> Response to hypoxia
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs
Cancer >> Signal transduction >> Other
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Stem Cells >> Signaling Pathways >> Notch >> Secreted
Signal Transduction >> Adapters >> Transmembrane
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
|
|
Relevance
|
ADAM17, also known as TNF alpha Convertase, or TACE, was first cloned from human epithelial cells. The ADAMs proteins are structurally similar, with a signal sequence, metalloprotease domain (inactive in some ADAMs), disintegrin domain, cystein rich domain, EGF like repeat, type I transmembrane and Cytoplasmic domain. A member of the metalloproteinase family containing disintegrin like domains (ADAMs), ADAM17 is known to process the TNF alpha trimer from the membrane attached precursor to the soluble form. ADAM17 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active, cleaving TNF precursor as well as TNF p75 receptor, myeloid precursor protein, amyloid plaque protein, L selectin and TGF alpha, making ADAM17 a "sheddase". ADAM17 contains a cleavage site for prohormone convertases (furin), and the majority of ADAM17 is secreted as activated enzyme, cleaved at the furin site. ADAM17 is known to associate with MAD2 via the Cytoplasmic domain, implying cellular regulation pathways. ADAM17 is expressed in a wide range of cells in culture. Three human ADAM17 sequences encode proteins with different cytoplasmic domains, as is the case for other ADAMs proteases. The 824 amino acid sequence encodes a 92,960 Dalton protein, the 807 AA sequence 91,003 Dalton, and the 694 AA sequence 78,543 Dalton predicted MW. Glycosylation and other post translational modifications increase the apparent molecular weight on PAGE gels. Mouse and rat ADAM17 sequences are both 827 AA, predicting 93,073 and 93,017 Dalton proteins, respectively. Reports have shown that ADAM17 expression in human breast tumor samples is an indicator of poor prognosis.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.01% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ADAM17 antibody - Activation site is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for ADAM17 antibody - Activation site images, references, products related to ab39163 and other tools.
|
| |
ADAM17 antibody - Activation site images:
|
 Immunocytochemistry/ Immunofluorescence - ADAM17 antibody - Activation site (ab39163)
The image shows ab39163 detecting mouse ADAM17 expressed in transfected HeLa cells. The cells were fixed in methanol and incubated with 10 µg/ml ab39163 for 1 hour at 20°C.
Image taken from anonymous Abreview submitted in February 2008
|
| |
Western blot - ADAM17 antibody - Activation site (ab39163)
ADAM17 antibody - Activation site (ab39163) at 1/4000 dilution + MDA-MB231 lysate
Secondary
Donkey anti-Rabbit at 1/10000 dilution
Predicted band size : 93 kDa
ab39163 detected ADAM17 protein in lysates of the highly invasive human breast cancer cell line MDA-MB231. ab39163 was incubated at a dilution of 1/4000 for 16 hours at 4°C in PBS + 5% Milk. For further details please refer to the Abreview. Image taken from an anonymous Abreview submitted in September 2007
|
| |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-ADAM17 antibody - Activation site(ab39163)
Ab39163 staining human normal colon tissue. Staining is localised to cellular membranes.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
|
| |
| |
|
Search PubMed (MEDLINE) for references to ADAM17
|
| |
ADAM17 antibody - Activation site - more information
|
Customer reviews (feedback) regarding ADAM17 antibody - Activation site |
Customer FAQs regarding ADAM17 antibody - Activation site |
Protocols for ADAM17 antibody - Activation site |
Price and availability of products related to ADAM17 antibody - Activation site |
| |
ADAM17 antibody - Activation site - related products:
|
|
|
| |
ADAM17 antibody - Activation site - other tools:
|
Contact Abcam with a Technical Enquiry about ADAM17 antibody - Activation site |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
