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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Overview
Product name
Anti-ADAM17 antibody - Aminoterminal end catalytic domain
See all ADAM17 products (14) ...
Description
Rabbit polyclonal to ADAM17 - Aminoterminal end catalytic domain
Tested applications
WB, ICC/IF, IPmore details
Cross reactivity
Reacts with
Rat, Human, Pig
Predicted to work with
Mouse
Immunogen
Synthetic peptide based on the amino end of the catalytic domain of human ADAM-17.
(Peptide available as ab41216.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs
Cancer >> Signal transduction >> Other
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Stem Cells >> Signaling Pathways >> Notch >> Secreted
Signal Transduction >> Adapters >> Transmembrane
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
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Immunocytochemistry/ Immunofluorescence - ADAM17 antibody - Aminoterminal end catalytic domain (ab28233)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab28233 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/5000.Predicted molecular weight: 120 kDa.(ab28233 recognizes a 120 kDa band in reduced Western blots of conditioned media or cell lysates, which is converted to a 55-60 kDa band. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Note: 1/1,000 when using colorimetric substrates such as BCIP/NBT, and 1/5,000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection.)
ICC/IF: Use a concentration of 10 µg/ml
IP: Use at an assay dependent concentration.
Target
Function
Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway.
Tissue specificity
Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Domain
Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
The precursor is cleaved by a furin endopeptidase.
Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791.
Cellular localization
Membrane.
Target information above from: UniProt accessionP78536
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibody
- A disintegrin and metalloproteinase domain 17 antibody
- ADA17_HUMAN antibody
see all
Database links
- Entrez Gene: 6868 Human
- Entrez Gene: 11491 Mouse
- Entrez Gene: 57027 Rat
- Omim: 603639 Human
- SwissProt: P78536 Human
- SwissProt: Q9Z0F8 Mouse
- SwissProt: Q9Z1K9 Rat
- Unigene: 404914 Human
see all
Anti-ADAM17 antibody - Aminoterminal end catalytic domain images:
Immunocytochemistry/ Immunofluorescence - ADAM17 antibody - Aminoterminal end catalytic domain (ab28233)
Methanol fixed Hela cells stained with ab28233 (10 µg/ml) for 1 hour at 22°C. Clear staining can be seen. For further details please refer to the Abreview
Image taken from an anonymous Abreview submitted in February 2008
Protocols
References for Anti-ADAM17 antibody - Aminoterminal end catalytic domain (ab28233)
ab28233 has not yet been referenced specifically in any publications.
Publishing research using ab28233? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"