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- Proteins and Peptides
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Product name
Anti-ADAM17 antibody
See all ADAM17 products (14) ...
Description
Goat polyclonal to ADAM17
Specificity
This antibody is expected to recognise isoform 1 (represented by NP_003174) and not isoform 2 (represented by NP_068604).
Tested applications
IP, ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chinese Hamster
Immunogen
Synthetic peptide: LQRQNRVDSKETEC, corresponding to C terminal amino acids 811-824 of Human ADAM17.
LQRQNRVDSKETE-C
Positive control
Hela Lysate
General notes
GenBank Accession Number - NP_003174
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Types of disease >> Neurodegenerative disease
Metabolism >> Pathways and Processes >> Metabolism processes >> Hypoxia
Cancer >> Cancer Metabolism >> Response to hypoxia
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs
Cancer >> Signal transduction >> Other
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Stem Cells >> Signaling Pathways >> Notch >> Secreted
Signal Transduction >> Adapters >> Transmembrane
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab13535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 125-130 kDa (predicted molecular weight: 95 , when glycosylated 130 kDa).Can be blocked with ADAM17 (C-term) peptide (human) (ab7881).
Target
Function
Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway.
Tissue specificity
Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Domain
Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
The precursor is cleaved by a furin endopeptidase.
Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791.
Cellular localization
Membrane.
Target information above from: UniProt accessionP78536
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibody
- A disintegrin and metalloproteinase domain 17 antibody
- ADA17_HUMAN antibody
see all
Database links
- Entrez Gene: 6868 Human
- Entrez Gene: 11491 Mouse
- Entrez Gene: 57027 Rat
- Omim: 603639 Human
- SwissProt: P78536 Human
- SwissProt: Q9Z0F8 Mouse
- SwissProt: Q9Z1K9 Rat
- Unigene: 404914 Human
see all
Anti-ADAM17 antibody images:
Western blot - ADAM17 antibody (ab13535)
Anti-ADAM17 antibody (ab13535) at 0.2 µg/ml + Hela lysate (RIPA buffer, 35µg total protein per lane)
Predicted band size : 95 , when glycosylated 130 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunocytochemistry/ Immunofluorescence - ADAM17 antibody (ab13535)
HT1080 cells incubated with ab13535 at 10µg/ml for 1 hour at 22°C. Cells were fixed for 20 minutes in 4% PFA in PBS then permeabilised in 0.1% Triton for 4 minutes
image taken from anonymous abreview
Immunoprecipitation - ADAM17 antibody (ab13535)
ab13535 Immunoprecipitate in human HEK293 whole cell lysate. 2 x 106 cells were used to prepare lysates and then incubated with primary antibody (diluted 1/250) and matrix (Protein G) for 16 hours at 4°C. For western blotting an Abcam ab28233 Rabbit polyclonal to ADAM17 was used at dilution at 1/5000.
This image is a courtesy of Anonymous Abreview.
References for Anti-ADAM17 antibody (ab13535)
ab13535 has not yet been referenced specifically in any publications.
Publishing research using ab13535? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"