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ab2051 has been referenced in 11 publications.
Publishing research using ab2051? Please let us know so that we can cite the reference in this datasheet
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All lanes : Anti-ADAM17 antibody (ab2051) at 1/500 dilution
Lane 1 : HeLa whole cell lysate with absence of blocking peptide
Lane 2 : Jurkat whole cell lysate with absence of blocking peptide
Lane 3 : HeLa whole cell lysate with
Lane 4 : Jurkat whole cell lysate with
Predicted band size : 93 kDa
80 to 130 kDa bands can be detected, which may represent mature protein, precursor, and glycosylated ADAM17.
Lane 1 : Anti-ADAM17 antibody (ab2051) at 0.5 µg/ml
Lane 2 : Unrelated antibody of the same subclass as ab2051.
Lane 1 : Human keratinocytes (HaCaT)- whole cell lysate from 20000 cells
Lane 2 : Human keratinocytes (HaCaT)- whole cell lysate from 20000 cells
Secondary
HRP conjugated goat polyclonal antibody
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 93 kDa
Observed band size : 100,125 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
This image is courtesy of an anonymous Abreview
ab2051 at 10µg/ml staining ADAM17 in Hela cells by ICC/IF
All lanes : Anti-ADAM17 antibody (ab2051) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : U937 cell lysate
Lane 3 : HT1080 cell lysate
Lane 4 : ES cell lysate
Lane 5 : L929 cell lysate
Lane 6 : CHO cell lysate
Lane 7 : 293T cell lysate
Lane 8 : ADAM17-overexpressing 293T cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
HRP-conjugated Goat anti-Rabbit polyclonal antibody at 1/4000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 93 kDa
Observed band size : 93 kDa
Additional bands at : 78 kDa (possible isoform).
Exposure time : 15 seconds
This image is courtesy of an anonymous Abreview.
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