ADAM17 antibody (ab2051)

Product Name 

ADAM17 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ADAM17

Immunogen 

Synthetic peptide, corresponding to amino acids 807-823 of Human ADAM17.
This sequence differs from those ofmouse and rat ADAM17 by one amino acid.

Reacts with 
(species key)

Hu, Ms, Rat

Epitope 

ASFKLQRQNRVDSKETE

Tested applications 
(see key)

Flow Cyt, ICC/IF, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use at an assay dependent dilution (PMID 19553533).
ICC/IF: Use at a concentration of 10 µg/ml.
Western blot (1:500 to 1:2000) detects bands of 80 to130 kDa bands, which may represent mature protein, precursor, and glycosylated TACE.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HeLa whole cell lysate, or Jurkat whole cell lysate.

Cellular localization 

Membrane; single pass type I membrane protein.

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs
Cancer >> Signal transduction >> Other
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Stem Cells >> Signaling Pathways >> Notch >> Secreted
Signal Transduction >> Adapters >> Transmembrane
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other

Relevance 

ADAM17, also known as TNF alpha Convertase, or TACE, was first cloned from human epithelial cells. The ADAMs proteins are structurally similar, with a signal sequence, metalloprotease domain (inactive in some ADAMs), disintegrin domain, cystein rich domain, EGF like repeat, type I transmembrane and Cytoplasmic domain. A member of the metalloproteinase family containing disintegrin like domains (ADAMs), ADAM17 is known to process the TNF alpha trimer from the membrane attached precursor to the soluble form. ADAM17 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active, cleaving TNF precursor as well as TNF p75 receptor, myeloid precursor protein, amyloid plaque protein, L selectin and TGF alpha, making ADAM17 a "sheddase". ADAM17 contains a cleavage site for prohormone convertases (furin), and the majority of ADAM17 is secreted as activated enzyme, cleaved at the furin site. ADAM17 is known to associate with MAD2 via the Cytoplasmic domain, implying cellular regulation pathways. ADAM17 is expressed in a wide range of cells in culture. Three human ADAM17 sequences encode proteins with different cytoplasmic domains, as is the case for other ADAMs proteases. The 824 amino acid sequence encodes a 92,960 Dalton protein, the 807 AA sequence 91,003 Dalton, and the 694 AA sequence 78,543 Dalton predicted MW. Glycosylation and other post translational modifications increase the apparent molecular weight on PAGE gels. Mouse and rat ADAM17 sequences are both 827 AA, predicting 93,073 and 93,017 Dalton proteins, respectively. Reports have shown that ADAM17 expression in human breast tumor samples is an indicator of poor prognosis.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

PBS with 0.02% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

TNFa Converting Enzyme.

See below for ADAM17 antibody images, references, products related to ab2051 and other tools.

ADAM17 antibody images:

References for ADAM17 antibody (ab2051)

  This product has been used in: (two most recent references)

Hanson EM et al. Myeloid-derived suppressor cells down-regulate L-selectin expression on CD4+ and CD8+ T cells. J Immunol 183:937-44 (2009). Flow Cyt. PubMed: 19553533

Katakowski M et al. Tumorigenicity of cortical astrocyte cell line induced by the protease ADAM17. Cancer Sci 100:1597-604 (2009). WB; Mouse. PubMed: 19515085

See all 5 publication references for this product.


If you publish research using ab2051 please let us know so that we can cite the reference on this datasheet.

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