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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Overview
Product name
Anti-ADAM17 antibody
See all ADAM17 products (14) ...
Description
Rabbit polyclonal to ADAM17
Tested applications
WB, ICC/IF, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide, corresponding to amino acids 807-823 of Human ADAM17. This sequence differs from those of mouse and rat ADAM17 by one amino acid.
Epitope
ASFKLQRQNRVDSKETE
Positive control
HeLa whole cell lysate, or Jurkat whole cell lysate.
General notes
TNFa Converting Enzyme.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.02% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs
Cancer >> Signal transduction >> Other
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Stem Cells >> Signaling Pathways >> Notch >> Secreted
Signal Transduction >> Adapters >> Transmembrane
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Predicted molecular weight: 93 kDa.(Detects bands of 80-130 kDa bands, which may represent mature protein, precursor, and glycosylated TACE.)
ICC/IF: Use a concentration of 10 µg/ml
Flow Cyt: Use at an assay dependent dilution. (PubMed: 19553533)
Target
Function
Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway.
Tissue specificity
Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Domain
Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
The precursor is cleaved by a furin endopeptidase.
Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791.
Cellular localization
Membrane.
Target information above from: UniProt accessionP78536
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibody
- A disintegrin and metalloproteinase domain 17 antibody
- ADA17_HUMAN antibody
see all
Database links
- Entrez Gene: 6868 Human
- Entrez Gene: 11491 Mouse
- Entrez Gene: 57027 Rat
- Omim: 603639 Human
- SwissProt: P78536 Human
- SwissProt: Q9Z0F8 Mouse
- SwissProt: Q9Z1K9 Rat
- Unigene: 404914 Human
see all
Anti-ADAM17 antibody images:
Western blot - ADAM17 antibody (ab2051)
All lanes : Anti-ADAM17 antibody (ab2051) at 1/500 dilution
Lane 1 : HeLa whole cell lysate with absence of blocking peptide
Lane 2 : Jurkat whole cell lysate with absence of blocking peptide
Lane 3 : HeLa whole cell lysate with ADAM17 peptide (ab7881)
Lane 4 : Jurkat whole cell lysate with ADAM17 peptide (ab7881)
Predicted band size : 93 kDa
80 to 130 kDa bands can be detected, which may represent mature protein, precursor, and glycosylated ADAM17.
Western blot - ADAM17 antibody (ab2051)
Lane 1 : Anti-ADAM17 antibody (ab2051) at 0.5 µg/ml
Lane 2 : Unrelated antibody of the same subclass as ab2051.
Lane 1 : Human keratinocytes (HaCaT)- whole cell lysate from 20000 cells
Lane 2 : Human keratinocytes (HaCaT)- whole cell lysate from 20000 cells
Secondary
HRP conjugated goat polyclonal antibody
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 93 kDa
Observed band size : 100,125 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
This image is courtesy of an anonymous Abreview
Immunocytochemistry/ Immunofluorescence - ADAM17 antibody (ab2051)
ab2051 at 10µg/ml staining ADAM17 in Hela cells by ICC/IF
Western blot - ADAM17 antibody (ab2051)
All lanes : Anti-ADAM17 antibody (ab2051) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : U937 cell lysate
Lane 3 : HT1080 cell lysate
Lane 4 : ES cell lysate
Lane 5 : L929 cell lysate
Lane 6 : CHO cell lysate
Lane 7 : 293T cell lysate
Lane 8 : ADAM17-overexpressing 293T cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
HRP-conjugated Goat anti-Rabbit polyclonal antibody at 1/4000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 93 kDa
Observed band size : 93 kDa
Additional bands at : 78 kDa (possible isoform).
Exposure time : 15 seconds
This image is courtesy of an anonymous Abreview.
References for Anti-ADAM17 antibody (ab2051)
This product has been referenced in:
- Thorp Eet al. Shedding of the Mer Tyrosine Kinase Receptor Is Mediated by ADAM17 Protein through a Pathway Involving Reactive Oxygen Species, Protein Kinase C{delta}, and p38 Mitogen-activated Protein Kinase (MAPK). J Biol Chem 286:33335-44 (2011).Read more (PubMed: 21828049) »
- Spin JMet al. Transcriptional profiling and network analysis of the murine angiotensin II-induced abdominal aortic aneurysm. Physiol Genomics 43:993-1003 (2011). IHC.Read more (PubMed: 21712436) »
See all 11 publications for this product
Publishing research using ab2051? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"